T-cell cytokines in chronic allergic eye disease
T-cell cytokines in chronic allergic eye disease
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DOI：
10.1016/s0091-6749(97)70279-3
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发表时间：
1997-12-01
影响因子：
14.2
通讯作者：
Lightman, SL
中科院分区：
文献类型：
--
作者：
Metz, DP;Hingorani, M;Lightman, SL
背景：慢性过敏性眼病的病理生理学不能仅用 I 型超敏反应来解释，T 细胞介导的炎症被强烈暗示为一种可能的额外机制。先前的研究表明，T-H2 样 T 细胞在一种慢性过敏性眼病中发挥着重要作用。 目的：本研究检测了不同临床组的慢性过敏性眼病受试者（即春季角结膜炎 [VKC]、特应性角结膜炎 [AKC] 和巨乳头状结膜炎 [GPC]）和正常对照受试者中 T 细胞的细胞因子谱。识别细胞因子信使 RNA (mRNA)，并使用双色免疫组织化学分析来证明定位于结膜 T 细胞的细胞因子免疫反应性。结果：与正常组织相比，过敏组织表达的 IL-3、IL-4 和 IL-5 mRNA 水平升高。 AKC 受试者的 IL-2 mRNA 表达显着高于 VKC (p = 0.004) 和 GPC (p = 0.02) 受试者。 T 细胞 IL-5 的免疫反应性在 VKC (p = 0.004)、GPC (p = 0.02) 和 AKC (p = 0.04) 受试者中比正常对照受试者更常见。然而，AKC 受试者中的 T 细胞 IFN-γ 蛋白表达高于 VKC (p = 0.01)、GPC (p = 0.01) 和对照受试者 (p = 0.005)。结论：这些结果显示，VKC 和 GPC 受试者中存在 T-H2 样 T 细胞细胞因子阵列，但细胞因子谱向 T-H1 样模式转变，可能是因为AKC 受试者的疾病。慢性过敏性眼病中这些重要的功能性 T 细胞变异可能对于理解临床特征和治疗反应的差异很重要。
Background: The pathophysiology of chronic allergic eye disease cannot be explained by type I hypersensitivity alone, and T cell-mediated inflammation has been strongly implicated as a possible additional mechanism. Previous studies suggested that T-H2-like T cells play an important role in one form of chronic allergic eye disease.Objectives: This study examined the cytokine profile of T cells in different clinical groups of subjects with chronic allergic eye disease (i.e., vernal keratoconjunctivitis [VKC], atopic keratoconjunctivitis [AKC], and giant papillary conjunctivitis [GPC]) and normal control subjects.Methods: In situ hybridization was used to identify cytokine messenger RNA (mRNA), and two-color immunohistochemical analysis was used to demonstrate cytokine immunoreactivity localizing to T cells in the conjunctiva.Results: Allergic tissue expressed increased levels of mRNA for IL-3, IL-4, and IL-5 when compared with normal tissue. There was significantly greater IL-2 mRNA expression in subjects with AKC than in those with VKC (p = 0.004) and those with GPC (p = 0.02). Immunoreactivity for T-cell IL-5 was present more frequently in subjects with VKC (p = 0.004), GPC (p = 0.02), and AKC (p = 0.04) than in normal control subjects. However, T-cell IFN-gamma protein expression was greater in subjects with AKC than in subjects with VKC (p = 0.01), GPC (p = 0.01), and control subjects (p = 0.005).Conclusions: These results show a T-H2-like T-cell cytokine array in subjects with VKC and GPC but a shift in cytokine profile toward a T-H1-like pattern, potentially because of differences in chronicity of the disorders, in subjects with AKC. These important functional T-cell variations in chronic allergic eye conditions are likely to be important in understanding differences in clinical characteristics and therapeutic responses.