Fundamental properties of oligo double-stranded DNA/single-walled carbon nanotube nanobiohybrids
Fundamental properties of oligo double-stranded DNA/single-walled carbon nanotube nanobiohybrids
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DOI：
10.1039/c0nr00145g
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发表时间：
2010-01-01
期刊：
影响因子：
6.7
通讯作者：
Nakashima, Naotoshi
中科院分区：
文献类型：
--
作者：
Yamamoto, Yuki;Fujigaya, Tsuyohiko;Nakashima, Naotoshi
描述了分别用20个碱基成对双链(Ds)DNA(Da)(20)/(Dt)(20)溶解的单壁碳纳米管(SWNTs)的基本性质，以及用于比较的寡聚DNA、腺嘌呤(Da)(20)和胸腺嘧啶(Dt)(20)的单链(Ss)(Ss)20-MERS。在这项研究中，使用尺寸排阻色谱(SEC)-高效液相色谱法从杂化水溶液中完全去除了未结合的寡聚DNA。每种溶液的SEC色谱图都有两个分离的峰，一个是游离的寡聚DNA，另一个是寡聚DNA/SWNT杂化的。较早洗脱组分(杂化组分)被分成四个大小不同的组分，然后通过重新进样这些组分来评价它们的稳定性。DsDNA/SWNTs和ssDNA/SWNTs杂化产物在水中具有较高的稳定性。这些结果使我们不必考虑结合的寡聚dsDNA或寡聚单链DNA从其与单壁碳纳米管的纳米杂化材料中解吸的问题，这对于寡聚DNA/SWNT纳米生物杂化材料在广泛的科学领域的应用具有显著的优势。我们还研究了不含相应游离寡核苷酸的分级寡聚DNA/单壁碳纳米管杂化材料的近红外吸收和光致发光(PL)光谱行为。
Fundamental properties of single-walled carbon nanotubes (SWNTs) that are individually dissolved using twenty base paired-double-stranded (ds) DNA, (dA)(20)/(dT)(20), as well as single-stranded (ss) twenty-mers of oligo DNAs, adenine (dA)(20) and thymine (dT)(20), for comparison are described. In this study, unbound oligo DNAs are fully removed from the hybrid aqueous solutions using size-exclusion chromatography (SEC)-HPLC. Each SEC chromatogram of the solutions shows two separated peaks; one is the free oligo DNAs and the others are the oligo DNA/SWNT hybrids. The earlier eluent fractions (the hybrids) are separated into four size-separated fractions, and then their stability is evaluated by the re-injection of the fractions. The chromatograms of the earlier eluent fractions are almost identical to those of the original ones even after storage for one month, indicating the high stability of the dsDNA/SWNTs and ssDNA/SWNTs hybrids in water. The results free us from considering the desorption of the bound-oligo dsDNA or oligo ssDNA from their nanohybrids with the SWNTs, which is of significant advantage to the utilization of oligo DNA/SWNT nanobiohybrids in wide areas of science. We also investigated the near-IR absorption and photoluminescence (PL) spectral behaviors of the fractionated oligo DNA/SWNTs hybrids not containing corresponding free oligo DNA.