Specific Host-Responsive Associations Between Medicago truncatula Accessions and Sinorhizobium Strains
Specific Host-Responsive Associations Between Medicago truncatula Accessions and Sinorhizobium Strains
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DOI：
10.1094/mpmi-01-17-0009-r
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发表时间：
2017-05-01
影响因子：
3.5
通讯作者：
Gruber, Veronique
中科院分区：
文献类型：
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作者：
Kazmierczak, Theophile;Nagymihaly, Marianna;Gruber, Veronique
豆科植物与根瘤菌相互作用形成固氮根瘤。豆科植物与根瘤菌的相互作用是特异性的，只有相容的根瘤菌和植物物种才能导致根瘤的形成。即使在相容的相互作用中，植物和细菌共生体的基因型也会影响根瘤功能和固氮活性的效率。模式豆科植物蒺藜苜蓿（Medicago truncatula）与中华根瘤菌属（Sinorhizobium）的几个种形成根瘤。然而，这些细菌菌株的效率高度可变。比较了苜蓿中华根瘤菌（Sinorhizobium meliloti）菌株Sm 1021、102F34、FSM-MA和苜蓿中华根瘤菌（Sinorhizobium medicae）菌株WSM 419对两种常用的苜蓿根瘤菌（M.蒺藜属材料A17和R108。相互作用的效率由多个参数决定。我们发现FSM-MA菌株对M.蒺藜属相反，A17-WSM419和R108 - 102F34组合获得了特异性高效相互作用。值得注意的是，广泛使用的Sm1021菌株在两种宿主上的表现都很弱。我们发现，Sm1021有效地诱导根瘤器官发生，但不能完全激活共生根瘤细胞的分化，解释其较弱的性能。这些结果将为利用这些M.这是一项重要的研究成果，特别是对蒺藜属种质的研究，特别是对蒺藜属种质后期阶段的研究。
Legume plants interact with rhizobia to form nitrogen-fixing root nodules. Legume-rhizobium interactions are specific and only compatible rhizobia and plant species will lead to nodule formation. Even within compatible interactions, the genotype of both the plant and the bacterial symbiont will impact on the efficiency of nodule functioning and nitrogen-fixation activity. The model legume Medicago truncatula forms nodules with several species of the Sinorhizobium genus. However, the efficiency of these bacterial strains is highly variable. In this study, we compared the symbiotic efficiency of Sinorhizobium meliloti strains Sm1021, 102F34, and FSM-MA, and Sinorhizobium medicae strain WSM419 on the two widely used M. truncatula accessions A17 and R108. The efficiency of the interactions was determined by multiple parameters. We found a high effectiveness of the FSM-MA strain with both M. truncatula accessions. In contrast, specific highly efficient interactions were obtained for the A17-WSM419 and R108-102F34 combinations. Remarkably, the widely used Sm1021 strain performed weakly on both hosts. We showed that Sm1021 efficiently induced nodule organogenesis but cannot fully activate the differentiation of the symbiotic nodule cells, explaining its weaker performance. These results will be informative for the selection of appropriate rhizobium strains in functional studies on symbiosis using these M. truncatula accessions, particularly for research focusing on late stages of the nodulation process.