Evaluation of chemiluminescent estradiol conjugates by using a surface plasmon resonance detector
Evaluation of chemiluminescent estradiol conjugates by using a surface plasmon resonance detector
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DOI：
10.1016/s0039-128x(00)00091-x
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发表时间：
2000-06-01
期刊：
影响因子：
2.7
通讯作者：
Yu, ZG
中科院分区：
文献类型：
--
作者：
Adamczyk, M;Chen, YY;Yu, ZG
合成了一系列17 β-雌二醇荧光探针。使用BIAcore表面等离子体共振仪上的单个E-2-类似物生物传感器表面评估溶液中抗E-2 Fab片段与探针相互作用的相对平衡解离常数（K-D）。结果显示抗体片段以高亲和力结合所有测试的荧光缀合物，仅显示对取代位点（C6相对于C7）、立体化学（α相对于β）或接头部分的较小偏好。(C)2000 Elsevier Science Inc. All rights reserved.
A series of chemiluminescent 17 beta-estradiol probes were synthesized. Relative equilibrium dissociation constants (K-D) for the interaction of an anti-E-2 Fab fragment for the probes in solution were evaluated using a single E-2-analog biosensor surface on a BIAcore surface plasmon resonance instrument. The results show the antibody fragment binds all chemiluminescent conjugates tested with high affinity showing only minor preferences for site of substitution (C6 versus C7), stereochemistry (alpha versus beta), or linker moiety. (C) 2000 Elsevier Science Inc. All rights reserved.