Evaluation of Titanium and Titanium Oxides as Chemo-affinity Sorbents for the Selective Enrichment of Organic Phosphates
Evaluation of Titanium and Titanium Oxides as Chemo-affinity Sorbents for the Selective Enrichment of Organic Phosphates
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DOI：
10.2116/analsci.23.1285
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发表时间：
2007-11
影响因子：
1.6
通讯作者：
A. Sano;Hiroshi Nakamura
中科院分区：
文献类型：
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作者：
A. Sano;Hiroshi Nakamura
研究了钛（Ti）和TiO、Ti_2O_3、Ti_3O_5、TiO_1.98以及TiO_2作为化学亲和吸附剂对有机磷的选择性富集作用。采用柱切换高效液相色谱（HPLC）系统，该系统由填充钛吸附剂的预柱（4.6 mm id × 10 mm）、阴离子交换分析柱和215 nm紫外检测器组成。以0.015%三氟乙酸（TFA）水溶液为上样液时，O -磷酸-l -酪氨酸（P-Tyr）、磷酸苯酯和苯膦酸均吸附在钛吸附剂上，回收率为60.9 ~ 102.9%。除磷酸盐以外的一些酸性化合物，如苯二羧酸（BDA）异构体，也被吸附到所有钛吸附剂上。为了提高对有机磷酸盐的选择性，我们使用Ti预柱、两种磷酸化肽[Ile-Ser(p)-Val-Arg （PP1）和Gln-Ile-Ser(p)-Val-Arg (PP2)]、p - tyr、BDA异构体和二谷氨酸（Glu-Glu）作为测试化合物，对不同的样品加载溶液进行了测试。在TFA、HClO_4、有机酸、硼酸和NaCl等载样溶液中，10mm硼酸中100mm NaCl的载样效果最好。PP1、PP2和P-Tyr的加样回收率分别为73.0、88.3和71.5%，而Glu-Glu和BDAs的加样回收率仅为10%以下。
Titanium (Ti) and TiO, Ti_2O_3, Ti_3O_5 and TiO_1.98 as well as TiO_2 have been evaluated as chemo-affinity sorbents for the selective enrichment of organic phosphates. A column-switching high-performance liquid chromatography (HPLC) system, constructed with a precolumn (4.6 mm i.d. × 10 mm) packed with the titanium sorbents, an anion-exchange analytical column and a UV detector (215 nm) was used. When an aqueous 0.015% trifluoroacetic acid (TFA) was used as a sample-loading solution, O -phospho-L-tyrosine (P-Tyr), phenyl phosphate and phenylphosphonic acid were adsorbed onto all of the titanium sorbents with recoveries of 60.9–102.9%. Some acidic compounds other than phosphates, such as benzenedicarboxylic acid (BDA) isomers, were also adsorbed onto all of the titanium sorbents. To improve the selectivity to organic phosphates, various sample-loading solutions were examined using a Ti precolumn, two phosphorylated peptides [Ile-Ser(p)-Val-Arg (PP1) and Gln-Ile-Ser(p)-Val-Arg (PP2)], P-Tyr, BDA isomers and diglutamic acid (Glu-Glu) as test compounds. Among the sample-loading solutions tested, such as TFA, HClO_4, organic acids, boric acid and NaCl, the use of 100 mM NaCl in 10 mM boric acid was found to be effective. The recoveries of PP1, PP2 and P-Tyr were 73.0, 88.3 and 71.5%, respectively, whereas those of Glu-Glu and BDAs were suppressed to only below 10%.