Bilateral mechanical and thermal hyperalgesia and tactile allodynia after chronic compression of dorsal root ganglion in mice
Bilateral mechanical and thermal hyperalgesia and tactile allodynia after chronic compression of dorsal root ganglion in mice
复制标题
小鼠背根神经节慢性受压后双侧机械和热痛觉过敏以及触觉异常性疼痛
DOI：
10.1007/s12264-011-1006-8
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发表时间：
2011-08-01
影响因子：
5.6
通讯作者：
Hu, San-Jue
中科院分区：
文献类型：
--
作者：
Chen, Rong-Gui;Kong, Wei-Wei;Hu, San-Jue
目的腰痛是临床上遇到的最难以解决的问题之一。模拟人类症状的动物模型是研究腰痛机制和可能的治疗应用的有价值的工具。随着基因技术在疼痛领域的发展，小鼠特异性基因突变的可能性为研究疼痛的特异性机制提供了有力的工具。本研究旨在建立慢性压迫背根神经节（CCD）小鼠模型，利用该模型中的基因突变促进慢性疼痛的研究。方法将细不锈钢棒分别插入L4和L5椎间孔，对小鼠L4和L5背根神经节进行慢性压迫。分别用von Frey纤维和辐射热刺激仪检测机械异常性痛和热痛觉过敏。结果CCD小鼠与CCD同侧后爪有明显的机械痛觉、热痛觉和触觉异常痛觉。此外，这种机械和热痛觉过敏以及触觉异常痛也被发现扩散到对侧后爪。结论：该模型与可能的基因改造相结合，将加强我们对腰痛潜在机制的认识。它也有利于开发新的治疗策略，疼痛和痛觉过敏后的脊髓损伤和其他疾病，影响背根神经节在人类。
ObjectiveLow back pain is one of the most inextricable problems encountered in clinics. Animal models that imitate symptoms in humans are valuable tools for investigating low back pain mechanisms and the possible therapeutic applications. With the development of genetic technology in pain field, the possibility of mutating specific genes in mice has provided a potent tool for investigating the specific mechanisms of pain. The aim of the present study was to develop a mouse model of chronic compression of dorsal root ganglion (CCD), in which gene mutation can be applied to facilitate the studies of chronic pain.MethodsChronic compression of L4 and L5 dorsal root ganglia was conducted in mice by inserting fine stainless steel rods into the intervertebral foramina, one at L4 and the other at L5. Mechanical allodynia and thermal hyperalgesia were examined with von Frey filaments and radiating heat stimulator, respectively.ResultsThe CCD mice displayed dramatic mechanical and thermal hyperalgesia as well as tactile allodynia in the hindpaw ipsilateral to CCD. In addition, this mechanical and thermal hyperalgesia as well as tactile allodynia was also found to spread to the contralateral hindpaw.ConclusionThis model, combined with the possible genetic modification, will strengthen our knowledge of the underlying mechanisms of low back pain. It also favors the development of new treatment strategies for pain and hyperalgesia after spinal injury and other disorders which affect the dorsal root ganglion in humans.