Durable, Stable, and Functional Nanopores Decorated by Self-Assembled Dipeptides
Durable, Stable, and Functional Nanopores Decorated by Self-Assembled Dipeptides
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DOI：
10.1021/acsami.0c00062
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发表时间：
2020-03-25
影响因子：
9.5
通讯作者：
Porath, Danny
中科院分区：
文献类型：
--
作者：
Karmi, Abeer;Sakala, Gowri Priya;Porath, Danny
纳米孔已经成为在单分子水平上检测和分析分子的重要工具。对固态纳米孔进行表面改性可以提高其耐久性和效率。肽是理想的表面修饰，因为它们允许通过合理设计其序列来定制多种性质。这里，氮化硅纳米孔被二肽层涂覆，其中L-3，4-二羟基苯丙氨酸（DOPA）残基是锚定元件，而另一个氨基酸部分是功能元件。DOPA与许多类型的表面紧密结合，并允许通过简单的浸渍进行表面的一步功能化。结果，涂覆的纳米孔的寿命从数小时增加到数月，并且电流稳定性相对于未涂覆的孔显著改善。这种改善是通过控制表面润湿性和电荷来实现的。肽包被的纳米孔可以用作敏感的传感器，其可以基于包被的肽的功能部分的选择来调节。此外，由于DNA与孔涂层的相互作用，涂层减缓了dsDNA的移位。
Nanopores have become an important tool for the detection and analysis of molecules at the single-molecule level. Surface modification of solid-state nanopores can improve their durability and efficiency. Peptides are ideal for surface modifications as they allow tailoring of multiple properties by a rational design of their sequence. Here, silicon nitride nanopores were coated by a dipeptide layer where a L-3,4-dihydroxyphenylalanine (DOPA) residue is the anchoring element and the other amino acid moiety is the functional element. DOPA binds tightly to many types of surfaces and allows a one-step functionalization of surfaces by simple immersion. As a result, the lifetime of coated nanopores increased from hours to months and the current- stability has significantly improved with respect to uncoated pores. This improvement is achieved by controlling the surface wettability and charge. Peptide-coated nanopores can be utilized as sensitive sensors that can be adjusted based on the choice of the functional moiety of the coated peptide. In addition, the coating slows down dsDNA translocation because of the DNA interaction with the pore coating.