Permanent colonization by Lactobacillus casei is hindered by the low rate of cell division in mouse gut
Permanent colonization by Lactobacillus casei is hindered by the low rate of cell division in mouse gut
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DOI：
10.1128/aem.70.2.670-674.2004
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发表时间：
2004-02-01
影响因子：
4.4
通讯作者：
Salminen, S
中科院分区：
文献类型：
--
作者：
Lee, YK;Ho, PS;Salminen, S
益生菌在肠道中停留时间长会延长其潜在的有益健康作用，并有助于定植。本研究利用5（和6）-羧基荧光素二乙酸琥珀酰氨基酯（cFDA-SE）测定了干酪乳杆菌在小鼠肠道内的定殖潜力，评估了其在肠道不同部位的倍增次数。BALB/c小鼠十二指肠、空肠、回肠和结肠表面覆盖的肠道水分分别为34.4 +/- 2.9 mg、58.8 +/- 6.8 mg、21.6 +/- 2.2 mg和8.0 +/- 1.0 mg。根据cfda - se标记的乳酸菌在肠道粘膜表面的残留浓度，估计饲喂的乳酸菌在十二指肠、空肠、回肠和结肠的平均被冲洗时间分别为3.98、1.55、1.34和2.48天。根据表面粘附细胞的残余荧光水平估计，乳酸菌在十二指肠、空肠、回肠和结肠中的平均倍增时间分别为4.10、4.78、4.56和5.59天。据估计，乳酸菌必须达到平均翻倍时间1.03 - 2.04天，才能更永久地定植在小鼠肠道的各个部分。
Long residence times of probiotics in the intestinal tract would prolong their potential beneficial health effects and assist colonization. This study investigated the colonization potential of Lactobacillus casei Shirota in mouse intestine by using 5 (and 6)-carboxyfluorescein diacetate, succinimidyl ester (cFDA-SE) for assessment of doubling times in different parts of the intestine. The amounts of intestinal water overlying the surfaces of the duodenum, jejunum, ileum, and colon in BALB/c mice were 34.4 +/- 2.9, 58.8 +/- 6.8, 21.6 +/- 2.2, and 8.0 +/- 1.0 mg, respectively. Based on the residual concentrations of cFDA-SE-labeled lactobacilli on intestinal mucosal surfaces, the average half times for the wash-out of lactobacilli fed were estimated at 3.98, 1.55, 1.34, and 2.48 days in the duodenum, jejunum, ileum, and colon, respectively. The average doubling times of the lactobacilli, estimated from the residual fluorescent levels of surface-adhered cells, were 4.10, 4.78, 4.56, and 5.59 days in the duodenum, jejunum, ileum, and colon, respectively. It is estimated that the lactobacilli would have to achieve an average doubling time of 1.03 to 2.04 days to colonize the various sections of the mouse intestinal tract more permanently.