Cytoplasmic ATPase involved in ferrous ion uptake from magnetotactic bacterium Magnetospirillum magneticum AMB-1
Cytoplasmic ATPase involved in ferrous ion uptake from magnetotactic bacterium Magnetospirillum magneticum AMB-1
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DOI：
10.1016/j.febslet.2007.06.047
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发表时间：
2007-07-24
期刊：
影响因子：
3.5
通讯作者：
Matsunaga, Tadashi
中科院分区：
文献类型：
--
作者：
Suzuki, Takeyuki;Okaimura, Yoshiko;Matsunaga, Tadashi
通过转座子诱变产生了具有位于ORF 4中的缺陷基因（基因ID：amb 4111）的磁致发光蛋白AMB-1（NMA 61）的非磁性突变体。ORF 4基因产物的生化特征表明，它定位于细胞质中并表现出ATP酶活性。NMA 61吸收铁的能力严重受损。培养基中的亚铁离子浓度随着野生型比NMA 61降低更多，而NMA 61的细胞质部分中的铁含量比野生型菌株低得多。这种胞质ATP酶是M.磁性AMB-1 (c)2007年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
A non-magnetic mutant of Magnetospirilluin magneticum AMB-1 (NMA61), harboring a defective gene located in ORF4 (gene ID: amb4111) was generated by transposon mutagenesis. Biochemical characterization of the gene product of ORF4 revealed that it was localized in the cytoplasm and displayed ATPase activity. The ability of NMA61 to take up iron was severely compromised. Ferrous ion concentration in the medium decreased more with the wild-type than with NMA61, while the iron content in the cytoplasmic fraction of NMA61 was much lower than the wild-type strain. This cytoplasmic ATPase is essential for iron trafficking within M. magneticum AMB-1. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.