Optimal Storage Conditions for Apheresis Research (OSCAR): a Biomedical Excellence for Safer Transfusion (BEST) Collaborative study
Optimal Storage Conditions for Apheresis Research (OSCAR): a Biomedical Excellence for Safer Transfusion (BEST) Collaborative study
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DOI：
10.1111/trf.14429
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发表时间：
2018-02-01
期刊：
影响因子：
2.9
通讯作者：
McKenna, David H.
中科院分区：
文献类型：
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作者：
Gniadek, Thomas J.;Garritsen, Henk S. P.;McKenna, David H.
细胞治疗产品经常在不同部位之间储存和运输。本研究的目的是确定储存温度、溶液和细胞浓度对非动员的外周血源性单核细胞（MNCs）的影响。研究设计和方法：本研究是一项多中心前瞻性研究，涉及健康志愿者，他们接受了非活动的单采MNC采集。产品在当地实验室处理，在5%的人血清白蛋白（HSA）或HypoThermosol FRS （HT; BioLife Solutions）中浓缩到100 × 10(6)或300 × 10(6)个有核细胞/mL。产品在室温（20-25℃）或冷藏温度（2-8℃）下保存，分别在0、24、48和72小时进行评估。检测NC和MNC浓度、细胞活力和CD3、CD4、CD8、CD14、CD19、CD25和CD56的流式细胞术分析。结果在所有测试条件下，细胞活力随时间的推移而下降。冷藏贮藏比室温贮藏保存的活力更大，特别是对于细胞浓度较高的产品。高细胞浓度的RT维持与CD14和cd4阳性细胞的相对损失相关，而其他标记物阳性细胞的浓度没有变化。最后，与HSA相比，HT有延迟性的pH降低；然而，两种解决方案之间的生存能力没有差异。结论：低细胞浓度(约为；100 × 10(6)个细胞/mL)，冷藏温度和高温储存溶液似乎是储存非动员的外周血源性MNC产品的最佳条件。
BACKGROUNDCell therapy products are often stored and transported between sites. The aim of this study was to determine the effect of storage temperature, solution, and cell concentration on nonmobilized, peripheral blood-derived mononuclear cells (MNCs).STUDY DESIGN AND METHODSThis was a multicenter prospective study involving healthy volunteers who underwent nonmobilized MNC collection by apheresis. Products were processed at local laboratories and concentrated to either 100 x 10(6) or 300 x 10(6) nucleated cells/mL in 5% human serum albumin (HSA) or HypoThermosol FRS (HT; BioLife Solutions). Products were stored at room temperature (RT; 20-25 degrees C) or refrigerated temperatures (2-8 degrees C) with assessment at 0, 24, 48, and 72 hours. NC and MNC concentration, viability, and flow cytometric analysis for CD3, CD4, CD8, CD14, CD19, CD25, and CD56 were measured.RESULTSViability decreased over time for all conditions tested. Refrigerated storage preserved viability greater than RT storage, especially for products with a higher cell concentration. RT maintenance with a high cell concentration was associated with a relative loss of CD14- and CD4-positive cells, whereas the concentration of cells positive for other markers tested did not vary. Finally, there was delayed decrease in pH when using HT compared with HSA; however, there was no difference in viability between the two solutions.CONCLUSIONLow cell concentrations (approx. 100 x 10(6) cells/mL), refrigerated temperatures, and HT storage solution appear to be the optimal conditions for storing nonmobilized, peripheral blood-derived MNC products.