Specificity in the interaction of HVA Ca2+ channel types with Ca2+-dependent AHPs and firing behavior in neocortical pyramidal neurons.
Specificity in the interaction of HVA Ca2+ channel types with Ca2+-dependent AHPs and firing behavior in neocortical pyramidal neurons.
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HVA Ca2 通道类型与 Ca2 依赖性 AHP 相互作用的特异性以及新皮质锥体神经元的放电行为。
DOI：
10.1152/jn.1998.79.5.2522
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Foehring,RC
中科院分区：
文献类型：
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作者：
Pineda,JC;Waters,RS;Foehring,RC
放大图片作者：Juan卡洛斯，Roberts S.沃茨和罗伯特C. Foehring。HVA Ca 2+通道类型与Ca 2+依赖性AHP相互作用的特异性和新皮质锥体神经元的放电行为。J. Neurophysiol.79：2522-2534，1998。细胞内记录和有机和无机钙离子通道阻滞剂被用于新皮层脑切片制备测试是否高电压激活（HVA）的钙离子通道的差异耦合到感觉运动新皮层锥体神经元的钙离子依赖性后超极化（AHPs）。在大多数情况下，尖峰复极不依赖于这些细胞中，虽然复极的最后阶段（快速AHP后）是敏感的N-型电流块。30%~ 60%的中后超极化（mAHP）和80%~ 90%的慢后超极化（sAHP）依赖于钙离子。基于特异性有机Ca 2+通道阻断剂（二氢吡啶类、ω-芋螺毒素GVIA、ω-agatoxin IVA和ω-芋螺毒素MVIIC）的作用，sAHP与N-、P-和Q-型电流偶联。P型电流耦合到mAHP。L-型电流不参与生成的AHP，但（与其他HVA电流）有助于内向电流，调节重复放电过程中的峰间间期。这些数据表明不同的功能后果调制的钙离子电流亚型。
Pineda, Juan Carlos, Roberts S. Waters, and Robert C. Foehring.Specificity in the interaction of HVA Ca2+channel types with Ca2+-dependent AHPs and firing behavior in neocortical pyramidal neurons.J. Neurophysiol.79: 2522–2534, 1998. Intracellular recordings and organic and inorganic Ca2+channel blockers were used in a neocortical brain slice preparation to test whether high-voltage–activated (HVA) Ca2+channels are differentially coupled to Ca2+-dependent afterhyperpolarizations (AHPs) in sensorimotor neocortical pyramidal neurons. For the most part, spike repolarization was not Ca2+dependent in these cells, although the final phase of repolarization (after the fast AHP) was sensitive to block of N-type current. Between 30 and 60% of the medium afterhyperpolarization (mAHP) and between ∼80 and 90% of the slow AHP (sAHP) were Ca2+dependent. Based on the effects of specific organic Ca2+channel blockers (dihydropyridines, ω-conotoxin GVIA, ω-agatoxin IVA, and ω-conotoxin MVIIC), the sAHP is coupled to N-, P-, and Q-type currents. P-type currents were coupled to the mAHP. L-type current was not involved in the generation of either AHP but (with other HVA currents) contributes to the inward currents that regulate interspike intervals during repetitive firing. These data suggest different functional consequences for modulation of Ca2+current subtypes.