Cartilage canals in human thyroid cartilage characterized by immunolocalization of collagen types I, II, pro-III, IV and X
Cartilage canals in human thyroid cartilage characterized by immunolocalization of collagen types I, II, pro-III, IV and X
复制标题
人甲状软骨中的软骨管以 I、II、pro-III、IV 和 X 型胶原蛋白的免疫定位为特征
DOI：
--
复制
发表时间：
1996
期刊：
影响因子：
--
通讯作者：
G. Simons
中科院分区：
文献类型：
--
作者：
H. Claassen;T. Kirsch;G. Simons
本研究采用针对I型、II型、前iii型、IV型和X型胶原的特异性抗体进行免疫标记，研究了人甲状腺软骨软骨管（即血管和结缔组织的软骨周内陷）和周围软骨基质的胶原组成。在儿童期和青春期早期，甲状腺软骨未发现软骨管，IV型胶原的免疫标记仅限于软骨膜血管的基底层成分。第一个免疫标记的IV型胶原，属于软骨管的血管，在两性中被发现大约在第二个十年结束；它局限于甲状软骨板的背侧。此时甲状软骨已经达到了最终的形状和大小。von Kossa染色显示，血管化先于矿化和骨化。与男性甲状软骨板相比，女性甲状软骨腹侧半部分即使高龄也未见IV型胶原免疫染色，未见骨化。软骨管内细胞的细胞外基质免疫染色显示I型胶原和前iii型胶原以及II型胶原阳性，表明软骨管内细胞具有成纤维细胞和软骨细胞的特性。靠近软骨管的肥大软骨细胞的细胞外基质对X型胶原表现出很强的免疫反应性。首先在软骨管附近检测到矿化，表明人甲状腺软骨的矿化始于软骨管附近的细胞外基质。
In this study the collagenous composition of cartilage canals in human thyroid cartilage, which are perichondral invaginations of blood vessels and connective tissue, and the surrounding cartilage matrix were investigated by immunolabelling with specific antibodies against type I, II, pro-III, IV and X collagen. During childhood and early adolescence no cartilage canals were detected in thyroid cartilage, and immunolabelling for type IV collagen was restricted to basal lamina components of blood vessels in the perichondrium. First immunolabelling for type IV collagen, belonging to blood vessels in cartilage canals, in both sexes was detected about the end of the second decade; it was localized in the dorsal part of the thyroid cartilage plate. At this time thyroid cartilage has already reached its final form and size. As revealed by von Kossa staining, vascularization preceded mineralization and ossification. In contrast to the male thyroid cartilage plate, no immunostaining for type IV collagen and no ossification was detected in the ventral half of female thyroid cartilage even in advanced age. The extracellular matrix of cells in cartilage canals showed positive immunostaining for collagen types I and pro-III as well as for collagen type II, indicating that the cells in the canal possess fibroblastic and chondrogenic properties. The extracellular matrix of hypertrophic chondrocytes adjacent to cartilage canals showed strong immunoreactivity for type X collagen. First mineralization was detected close to cartilage canals, suggesting that mineralization in human thyroid cartilage starts in the extracellular matrix adjacent to cartilage canals.