Single cell origin of multilineage colonies in culture. Evidence that differentiation of multipotent progenitors and restriction of proliferative potential of monopotent progenitors are stochastic processes.
Single cell origin of multilineage colonies in culture. Evidence that differentiation of multipotent progenitors and restriction of proliferative potential of monopotent progenitors are stochastic processes.
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培养物中多谱系集落的单细胞起源。
DOI：
10.1172/jci111645
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Civin,CI
中科院分区：
文献类型：
--
作者：
Leary,AG;Ogawa,M;Strauss,LC;Civin,CI
在本文中，我们报告的分析，在人类造血集落的分化来自一个单一的细胞。将脐带血单核细胞和淘选的My-10抗原阳性骨髓和脐带血细胞接种在含有促红细胞生成素和条件培养基的甲基纤维素培养基中。最初，我们进行了定位研究，以确定候选集落形成细胞。随后，使用显微操作器，我们将单个细胞单独转移到35 mm培养皿中，用于分析集落形成。通过鉴定May-Grunwald-Giemsa染色制备物中的所有细胞来确定殖民地的细胞组成。在150个单个候选细胞中，63个产生集落。单系集落的发生率包括19个红系集落、17个单核-巨噬细胞集落和9个嗜酸性粒细胞集落。有18个混合造血集落由不同组合的两个、三个、四个和五个谱系的细胞组成。在某些情况下，我们注意到一个谱系占优势，而在第二或第三个谱系中存在非常小的细胞群体。这些结果为人类多系造血集落的单细胞起源提供了证据，并与人类干细胞分化的随机模型相一致。它们还表明定向祖细胞增殖潜力的限制是一个随机过程。图像
In this paper, we report analysis of differentiation in human hemopoietic colonies derived from a single cell. Cord blood mononulear cells and panned My-10 antigen-positive bone marrow and cord blood cells were plated in methylcellulose medium containing erythropoietin and conditioned medium. Initially, we performed mapping studies to identify candidate colony-forming cells. Subsequently, using a micromanipulator, we transferred single cells individually to 35-mm dishes for analysis of colony formation. Cellular composition of the colony was determined by identifying all of the cells in the May-Grunwald-Giemsa stained preparation. Of 150 single candidate cells replated, 63 produced colonies. The incidences of single lineage colonies included 19 erythroid, 17 monocyte-macrophage, and 9 eosinophil colonies. There were 18 mixed hemopoietic colonies consisting of cells in two, three, four, and five lineages in varying combinations. In some instances, we noted the predominance of one lineage and the presence of very small populations of cells in a second or third lineage. These results provide evidence for the single-cell origin of human multilineage hemopoietic colonies, and are consistent with the stochastic model of stem cell differentiation in man. They also indicate that restriction of the proliferative potential of committed progenitors is a stochastic process.Images