DIFFERENTIAL EXPRESSION OF THE MESSENGER-RNAS FOR THE SOLUBLE AND MEMBRANE-BOUND FORMS OF RABBIT CYTOCHROME-B5
DIFFERENTIAL EXPRESSION OF THE MESSENGER-RNAS FOR THE SOLUBLE AND MEMBRANE-BOUND FORMS OF RABBIT CYTOCHROME-B5
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DOI：
10.1016/0167-4781(93)90274-h
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发表时间：
1993-02-20
期刊：
影响因子：
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通讯作者：
STEGGLES, AW
中科院分区：
文献类型：
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作者：
GIORDANO, SJ;STEGGLES, AW
从各种兔组织中提取总RNA，并逆转录用于聚合酶链反应技术。使用设计用于扩增膜结合的肝细胞色素b5 cDNA的引物，观察到两种大小的产物。两者都与放射性标记的肝细胞色素b5探针强烈杂交。测序证实，这两种类型的cDNA产物编码的膜结合和可溶性形式的b5。在肺、胆囊和肾上腺以及网织红细胞和骨髓中检测到对应于可溶性细胞色素的信使RNA。这是一个出乎意料的发现，因为该蛋白质仅从红细胞中分离出来。与此相反，膜结合细胞色素b5 mRNA检测在所有组织中测试，这表明相应的蛋白质是无处不在的组织分布。
Total RNA was extracted from a variety of rabbit tissues and reverse transcribed for use in the polymerase chain reaction technique. Using primers designed to amplify the membrane-bound liver cytochrome b5 cDNA, products of two sizes were observed. Both hybridized strongly to a radiolabelled liver cytochrome b5 probe. Sequencing confirmed that the two types of cDNA product encoded the membrane-bound and the soluble forms of b5. Messenger RNA corresponding to the soluble cytochrome was detected in the lung, gallbladder and the adrenal gland, as well as in reticulocytes and bone marrow. This was an unexpected finding since the protein has been isolated only from erythrocytes. In contrast, membrane-bound cytochrome b5 mRNA was detected in all tissues tested, suggesting that the corresponding protein is ubiquitous in tissue distribution.