The complete amino acid sequence of human brain-derived acidic fibroblast growth factor.
The complete amino acid sequence of human brain-derived acidic fibroblast growth factor.
复制标题
人脑源性酸性成纤维细胞生长因子的完整氨基酸序列。
DOI：
10.1016/s0006-291x(86)80540-x
复制
发表时间：
1986
影响因子：
3.1
通讯作者：
Thomas,KA
中科院分区：
文献类型：
--
作者：
Gimenez-Gallego,G;Conn,G;Hatcher,VB;Thomas,KA
酸性成纤维细胞生长因子是一种有效的促有丝分裂剂，可用于培养多种细胞，包括血管内皮细胞，并且是体内血管生成素。人脑源性酸性成纤维细胞生长因子的氨基酸全序列是通过对全蛋白的氨基末端序列分析和羧肽酶A消化以及胰蛋白酶、金黄色葡萄球菌V8蛋白酶和溴化氰裂解产生的肽的序列分析确定的。人蛋白中存在潜在的Asn-Gly-Ser糖基化序列。将完整的氨基酸序列与从牛脑纯化的等效蛋白质的氨基酸序列进行比较。
Acidic fibroblast growth factor is a potent mitogen for a variety of cells in culture, including vascular endothelial cells, and is angiogenicin vivo. The complete amino acid sequence of human brain-derived acidic fibroblast growth factor has been determined from amino terminal sequence analysis and carboxypeptidase A digestion of the whole protein and sequence analyses of peptides generated by tryptic,Staphylococcus aureusV8 protease and cyanogen bromide cleavages. A potential Asn-Gly-Ser glycosylation sequence is present in the human protein. The complete amino acid sequence is compared to that of the equivalent protein purified from bovine brain.