Vasopressin transiently stimulates phospholipase C activity in cultured rat hepatocytes.
Vasopressin transiently stimulates phospholipase C activity in cultured rat hepatocytes.
复制标题
加压素可短暂刺激培养的大鼠肝细胞中的磷脂酶 C 活性。
DOI：
10.1016/0167-4889(89)90165-1
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Fain,JN
中科院分区：
文献类型：
--
作者：
Pittner,RA;Fain,JN
加压素刺激原代培养的大鼠肝细胞磷脂酶C活性，在无血清条件下维持18-24小时。在胆酸盐、脱氧胆酸盐和氯化钠存在下，用外源[3 H]磷脂酰肌醇4，5-二磷酸([3 H]PIP2)测定可溶性和膜相关磷脂酶C的活性。将肝细胞暴露于加压素(100 NM)中，可使膜相关磷脂酶C和可溶性磷脂酶C活性分别增加30%和40%。然而，到了15岁的S，这种刺激已经消失了。在肝细胞中加入加压素，先前用[~3H]肌醇标记，在5 S内刺激肌醇磷酸生成，但在5分钟孵育后几乎看不到进一步的增加。这些结果表明，加压素迅速刺激可溶性和膜相关磷脂酶C的活性。
Vasopressin stimulated phospholipase C activity in primary cultures of rat hepatocytes maintained for 18–24 h under serum free conditions. Soluble and membrane-associated phospholipase C activity was determined using exogenous [3H]phosphatidylinositol 4,5-bisphosphate ([3H]PIP2) in the presence of cholate, deoxycholate and NaCl. Exposure of hepatocytes for 5 s to vasopressin (100 nM) stimulated both membrane-associated and soluble phospholipase C activity by 30% and 40%, respectively. However, by 15 s this stimulation had disappeared. Addition of vasopressin to hepatocytes, previously labelled with [3H]inositol, stimulated inositol phosphate production within 5 s, but little further increase was seen over a 5-min incubation. These results indicate that vasopressin rapidly stimulates both soluble and membrane-associated phospholipase C activity.