SPECIFICITY OF COAGGREGATION REACTIONS BETWEEN HUMAN ORAL STREPTOCOCCI AND STRAINS OF ACTINOMYCES-VISCOSUS OR ACTINOMYCES-NAESLUNDII
SPECIFICITY OF COAGGREGATION REACTIONS BETWEEN HUMAN ORAL STREPTOCOCCI AND STRAINS OF ACTINOMYCES-VISCOSUS OR ACTINOMYCES-NAESLUNDII
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DOI：
10.1128/iai.24.3.742-752.1979
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发表时间：
1979-01-01
影响因子：
3.1
通讯作者：
MCINTIRE, FC
中科院分区：
文献类型：
--
作者：
CISAR, JO;KOLENBRANDER, PE;MCINTIRE, FC
放线菌和链球菌细胞之间的共聚集反应经常发生，当人类菌株A。viscosus或A.内氏链球菌与人血链球菌或链球菌分离株混合。但很少与其他口腔放线菌和链球菌。两组放线菌和4组链球菌通过它们的共聚集反应的模式和β-葡聚糖的能力来定义。连接的半乳糖苷（即，乳糖）来逆转这些反应。共聚集通过以下3种细胞-细胞相互作用中的一种发生：共聚集通过加热链球菌而不是放线菌而被阻断，并且不被乳糖逆转;共聚集通过加热放线菌而不是链球菌而被阻断，并且被乳糖逆转;以及共聚集仅通过加热两种细胞类型而被阻断。后一种反应是前两种反应的结合，因为乳糖逆转了加热的链球菌和未加热的放线菌之间的共聚集，但没有逆转未加热的链球菌和加热的放线菌之间的共聚集。可以热灭活的细胞通过氨基乙酰化或蛋白酶消化灭活，而不受热影响的细胞不会被这些处理灭活。每种共聚集反应都依赖于Ca ~（2+），对葡聚糖酶处理不敏感。人源A. viscosus和A.内氏酵母菌可与S. sanguis和S.缓解是通过一种细胞类型上的蛋白质或糖蛋白受体与另一种细胞类型上的碳水化合物之间的特异性细胞表面相互作用系统实现的。
Co-aggregation reactions between actinomycete and streptococcal cells occurred frequently when human strains of A. viscosus or A. naeslundii were mixed with human isolates of Streptococcus sanguis or S. mitis but were infrequent with other oral actinomycetes and streptococci. Two groups of actinomycetes and 4 groups of streptococci were defined by the patterns of their co-aggregation reactions and by the ability of .beta.-linked galactosides (i.e., lactose) to reverse these reactions. Co-aggregations occurred by 1 of the following 3 kinds of cell-cell interactions: co-aggregation that was blocked by heating the Streptococcus but not the actinomycete and was not reversed by lactose; co-aggregation that was blocked by heating the actinomycete but not the Streptococcus and was reversed by lactose; and co-aggregation that was blocked only by heating both cell types. The latter reaction was a combination of the 1st two since lactose reversed co-aggregation between heated streptococci and unheated actinomycetes but did not reverse co-aggregations between unheated streptococci and heated actinomycetes. Cells that could be heat-inactivated were inactivated by amino group acetylation or protease digestion, whereas cells that were unaffected by heat were not inactivated by these treatments. Co-aggregation reactions of each kind were Ca2+ dependent and insensitive to dextranase treatment. Human strains of A. viscosus and A. naeslundii may co-aggregate with strains of S. sanguis and S. mitis by a system of specific cell surface interactions between protein or glycoprotein receptors on 1 cell type and carbohydrates on the other type.