Genome-wide analysis of Nilaparvata lugens nymphal responses to high-density and low-quality rice hosts
Genome-wide analysis of Nilaparvata lugens nymphal responses to high-density and low-quality rice hosts
复制标题
褐飞虱若虫对高密度和低质量水稻寄主的反应的全基因组分析
DOI：
10.1111/j.1744-7917.2012.01571.x
复制
发表时间：
2013-12-01
期刊：
影响因子：
4
通讯作者：
Li, Jun-Sheng
中科院分区：
文献类型：
--
作者：
Hu, Ding-Bang;Luo, Bing-Qing;Li, Jun-Sheng
褐飞虱是水稻上的重要害虫。本研究利用水稻黄熟期和较高的虫口密度，构建了抗褐飞虱的逆境生存条件。同时，利用水稻分蘖期和低密度的群体，建立了一个适宜的生存条件（SSC）作为对照。使用Solexa/Illumina测序来鉴定响应ASC的BPH若虫的基因。与SSC相比，ASC能显著延长BPH成虫的发育历期，显著降低BPH成虫的短翅率。共获得2544个差异表达基因（DEG），并通过BLASTx、Gene Ontology和KEGG Orthology进行分析。基因本体分析表明，DEG主要涉及细胞、细胞部分、细胞过程、结合、催化、细胞器和代谢过程等类别。将1138个具有酶委员会编号的DEG分配到不同的代谢途径。最大的集群是神经退行性疾病（137，12.0%），其次是碳水化合物代谢（113，9.9%），氨基酸代谢（94，8.3%），核苷酸代谢（76，6.7%）、能量代谢（64，5.6%）、翻译（60，5.3%）、脂质代谢（58，5.1%）和折叠、分选和降解（52，4.6%）。采用实时荧光定量聚合酶链反应技术分析了11个DEG在BPH若虫发育过程中的表达谱。这11个基因至少在一个发育阶段表现出ASC和SSC之间的差异表达。在这项研究中确定的DEG提供了BPH如何重新配置其基因表达谱，以适应过度拥挤和低质量的主机的分子证据。
The brown planthopper (BPH) Nilaparvata lugens is an economically important pest on rice plants. In this study, the higher population density and yellow-ripe stage of rice plants were used to construct adverse survival conditions (ASC) against BPH nymphs. Simultaneously, the low population density and tillering stage of rice plants were used to establish a suitable survival condition (SSC) as a control. Solexa/Illumina sequencing was used to identify genes of BPH nymphs responding to ASC. Significantly longer duration development of BPH nymphs and significantly lower brachypterous ratio of BPH adults were observed by ASC compared with SSC. A total of 2544 differentially expressed genes (DEGs) were obtained and analyzed by BLASTx, Gene Ontology and KEGG Orthology. Gene ontology analysis revealed that the DEGs were mainly involved in categories of cell, cell part, cellular process, binding, catalytic, organelle and metabolic processes. 1 138 DEGs having enzyme commission numbers were assigned to different metabolic pathways. The largest clusters were neurodegenerative diseases (137, 12.0%), followed by carbohydrate metabolism (113, 9.9%), amino acid metabolism (94, 8.3%), nucleotide metabolism (76, 6.7%), energy metabolism (64, 5.6%), translation (60, 5.3%), lipid metabolism (58, 5.1%), and folding, sorting and degradation (52, 4.6%). Expressing profile of 11 DEGs during eight nymphal developmental stages of BPH were analyzed by quantitative real-time polymerase chain reaction. The 11 genes exhibited differential expression between ASC and SSC during at least one developmental stage. The DEGs identified in this study provide molecular proof of how BPH reconfigures its gene expression profile to adapt to overcrowding and low-quality hosts.