Detection and characterisation of group A rotavirus in asymptomatic piglets in southern Ireland
Detection and characterisation of group A rotavirus in asymptomatic piglets in southern Ireland
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DOI：
10.1007/s00705-010-0713-1
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发表时间：
2010-08-01
影响因子：
2.7
通讯作者：
O'Shea, H.
中科院分区：
文献类型：
--
作者：
Collins, P. J.;Martella, V.;O'Shea, H.
猪 A 组轮状病毒 (GARV) 是仔猪肠炎的病原体，也是人类 GARV 多样化的遗传物质的大量库。猪病毒遗传异质性信息的积累对于轻松表征不寻常的人类毒株至关重要。 2005 年至 2007 年间，对爱尔兰 4 个猪群的 4-5 至 8-9 周龄无症状猪采集的 292 份粪便样本进行了筛查，结果有 19 份（6.5%）样本通过逆转录 PCR (RT-PCR) 检测出 GARV 阳性。对菌株进行分子表征，以整理有关 VP7 和部分 VP4 外衣壳基因的数据。通过VP7基因序列分析，爱尔兰毒株被鉴定为G2、G4、G5、G9和G11病毒。 G11 毒株与其他人类和猪 G11 毒株密切相关，而 G2 毒株与最近在欧洲和南亚发现的猪 G2 病毒相似。 G4 品系与其他 G4 人类和动物品系关系较远，构成独立的 G4 VP7 谱系。对 G5 毒株的分析表明，它们与精选的 G5 人类和猪毒株相似，而 G9 毒株与其他猪 G9 病毒相似。通过对VP4的VP8*片段进行序列分析，爱尔兰病毒被定性为P[6]、P[7]、P[13]、P[13]/[22]、P[26]和P[32]。
Porcine group A rotaviruses (GARV) are causative agents of enteritis in piglets and are a large reservoir of genetic material for the diversification of human GARVs. Accumulation of information on the genetic heterogeneity of porcine viruses is pivotal for readily characterising unusual human strains. Screening of 292 fecal samples, collected from 4-5- to 8-9-week-old asymptomatic pigs from four herds in Ireland between 2005 and 2007 resulted in 19 (6.5%) samples testing positive by reverse-transcription PCR (RT-PCR) for GARV. The strains were molecularly characterized to collate data on the VP7 and partial VP4 outer capsid genes. By sequence analysis of the VP7 gene, the Irish strains were identified as G2, G4, G5, G9 and G11 viruses. The G11 strains were closely related to other human and porcine G11 strains, while the G2 strains resembled porcine G2 viruses detected recently in Europe and southern Asia. The G4 strains were distantly related to other G4 human and animal strains, constituting a separate G4 VP7 lineage. Analysis of the G5 strains revealed that they were similar to a selection of G5 human and porcine strains, while the G9 strains resembled other porcine G9 viruses. By sequence analysis of the VP8* fragment of the VP4, the Irish viruses were characterised as P[6], P[7], P[13], P[13]/[22], P[26] and P[32].