The short-term effects of delayed application of electric fields in the damaged rodent spinal cord.
The short-term effects of delayed application of electric fields in the damaged rodent spinal cord.
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DOI：
10.1097/00006123-198907000-00012
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发表时间：
1989-07
期刊：
影响因子：
4.8
通讯作者：
M. Politis;M. Zanakis
中科院分区：
文献类型：
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作者：
M. Politis;M. Zanakis
以往的研究表明，对大鼠脊髓挫伤施加电场可以在损伤/治疗后3周内部分恢复功能。研究是否可以在延迟治疗后建立类似的功能恢复。大鼠脊髓挫伤，10天后恢复正常。当时，一个提供3微安直流电的刺激器被施加到脊髓的背部，使阴极朝向病变的吻部或尾部。非激活刺激物也作为对照。然后，每周在斜面上对大鼠进行行为和临床评估，最长可达3周，然后处死大鼠进行组织学评估。结果表明，“阴极嘴”和“阳极嘴”两组在斜面上的表现均显著好于“无电流”组。阴极嘴和阳极嘴组动物也表现出更好的运动能力。在积极治疗组中，病变吻端背侧FUNICULI的轴突数量在统计学上也明显多于未治疗的对照组。阴极嘴组动物在病变部位附近显示出更多可存活的神经元胞体。这些数据表明，即使在延迟治疗后，电场也可能促进哺乳动物脊髓的功能恢复和再生。
Previous studies have indicated that the application of electrical fields to the contused rat spinal cord could result in a partial return of function within 3 weeks after injury/treatment. Whether similar functional recovery could be established after a delay in the treatment was investigated. Rat spinal cords were contused and allowed to recover untreated for 10 days. At that time, a stimulator delivering 3 microA of direct current was applied to the dorsal portion of the cord such that the cathode was oriented either rostral or caudal to the lesion. Inactive stimulators were also used as controls. Rats were then assessed behaviorally and clinically at weekly intervals up to 3 weeks on an inclined plane and then killed for histological assessments. The results indicate that both the "cathode rostral" and "anode rostral" groups performed statistically significantly better on the inclined plane than the "no current" group. The cathode rostral and anode rostral group animals also demonstrated superior motor abilities. The number of axons in the dorsal funiculi rostral to the lesion in the actively treated groups were also statistically significantly greater than no current controls. The cathode rostral group animals demonstrated a greater number of viable neuronal cell bodies near the site of the lesion. These data suggest that electric fields may facilitate functional recovery and regeneration in the mammalian spinal cord, even after a delay in such treatment.