NMR AND MOLECULAR MODELING INVESTIGATIONS OF THE NEUROPEPTIDE BRADYKININ IN 3 DIFFERENT SOLVENT SYSTEMS - DMSO, 9/1 DIOXANE/WATER, AND IN THE PRESENCE OF 7.4 MM LYSO PHOSPHATIDYLCHOLINE MICELLES
NMR AND MOLECULAR MODELING INVESTIGATIONS OF THE NEUROPEPTIDE BRADYKININ IN 3 DIFFERENT SOLVENT SYSTEMS - DMSO, 9/1 DIOXANE/WATER, AND IN THE PRESENCE OF 7.4 MM LYSO PHOSPHATIDYLCHOLINE MICELLES
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DOI：
10.1002/bip.360340504
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发表时间：
1994-05-01
期刊：
影响因子：
2.9
通讯作者：
HICKS, RP
中科院分区：
文献类型：
--
作者：
YOUNG, JK;HICKS, RP
线性九肽激素缓激肽（Arg（1）-Pro（2）-pro（3）-Gly（4）-Phe（5）-Ser（6）-pro（7）-Phe（8）Arg（9））直接或间接参与多种生理过程，特别是疼痛和痛觉超敏。另外的证据表明，缓激肽在炎症反应、哮喘、脓毒症和鼻病毒感染相关症状中也起重要作用。长期以来，人们推测缓激肽C-末端的β-转角在神经肽的生物活性中起主要作用。缓激肽在三个截然不同的局部化学环境中，DMSO，9：1二氧六环/水，并在7.4 mM的裂解磷脂酰胆碱胶束的存在下，β-转角形成潜力，研究了使用二维home-NMR实验加上模拟退火计算。这些研究的结果表明，在所有三个系统中，C-末端的残基6-9采用非常相似的β-转角样结构。这些结果表明缓激肽C端的β转角是受体识别和结合的重要二级结构特征。(C)1994年，John Wiley and Sons，Inc.
The linear nonapeptide hormone bradykinin (Arg(1)-Pro(2)-pro(3)-Gly(4)-Phe(5)-Ser(6)-pro(7)-Phe(8) Arg(9)) is involved, either directly or indirectly, in a wide variety of physiological processes, particularly pain and hyperanalgesia. Additional evidence suggests that bradykinin also plays a major role in inflammatory response, asthma, sepsis, and symptoms associated with the rhinoviral infection. It has long been speculated that a beta-turn the C-terminus of bradykinin plays a major role in the biological activity of the neuropeptide. The beta-turn forming potential of bradykinin in three vastly different local chemical environments, DMSO, 9 : 1 dioxane/water, and in the presence of 7.4 mM lyse phosphatidylcholine micelles, was investigated using two-dimensional homonuclear nmr experiments coupled with simulated annealing calculations. The results of these investigations show that in all three systems residues 6-9 of the C-terminus adopt very similar beta-turn like structures. These results suggest that the beta-turn at the C-terminus of bradykinin is an important secondary structural feature for receptor recognition and binding. (C) 1994 John Wiley and Sons, Inc.