Brca1 and Brca2 expression patterns in mitotic and meiotic cells of mice.
Brca1 and Brca2 expression patterns in mitotic and meiotic cells of mice.
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DOI：
10.1038/sj.onc.1201506
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发表时间：
1998-01-08
期刊：
影响因子：
8
通讯作者：
Davis, BJ
中科院分区：
文献类型：
--
作者：
Blackshear, PE;Goldsworthy, SM;Davis, BJ
乳腺癌易感基因Brca1和Brca2的小鼠同源物在体外以细胞周期依赖的方式表达，并且似乎受到相似或重叠的途径的调节。因此，我们比较了Brca1和Brca2 mRNA在小鼠胚胎发生、乳腺发育过程中有丝分裂和减数分裂细胞以及包括睾丸在内的成年组织中的体内非同位素原位杂交表达模式，在卵巢和卵巢改变的情况下，Brca 1和Brca 2在胚胎的增殖细胞中一致地表达，并且在经历形态发生的乳腺和大多数成年组织中一致地表达。Brca 1和Brca 2的表达模式与增殖细胞核抗原的定位相关，增殖细胞核抗原是增殖活性的指标。在卵巢中，Brca1和Brca2在卵母细胞、颗粒细胞和卵泡膜细胞中的表达具有相似的非依赖性。在睾丸中，Brca1和Brca2在有丝分裂期精原细胞和减数分裂早期精母细胞中表达，对青春期前小鼠睾丸的北方分析显示，相对于Brca 1，精原细胞中Brca 2表达的时间进程延迟。因此，虽然Brca1和Brca2在大多数增殖组织中具有一致的细胞特异性表达模式，但这些观察结果表明它们在减数分裂过程中可能具有不同的作用。
The mouse homologues of the breast cancer susceptibility genes, Brca1 and Brca2, are expressed in a cell cycle-dependent fashion in vitro and appear to be regulated by similar or overlapping pathways, Therefore, we compared the non isotopic in situ hybridization expression patterns of Brca1 and Brca2 mRNA in vivo in mitotic and meiotic cells during mouse embryogenesis, mammary gland development, and in adult tissues including testes, ovaries, and hormonally altered ovaries, Brca1 and Brca2 are expressed concordantly in proliferating cells of embryos, and the mammary gland undergoing morphogenesis and in most adult tissues, The expression pattern of Brca1 and Brca2 correlates with the localization of proliferating cell nuclear antigen, an indicator of proliferative activity, In the ovary, Brca1 and Brca2 exhibited a comparable hormone-independent pattern of expression in oocytes, granulosa cells and thecal cells of developing follicles, In the testes, Brca1 and Brca2 were expressed in mitotic spermatogonia and early meiotic prophase spermatocytes, Northern analyses of prepubertal mouse testes revealed that the time course of Brca2 expression was delayed in spermatogonia relative to Brca1. Thus, while Brca1 and Brca2 share concordant cell-specific patterns of expression in most proliferating tissues, these observations suggest that they may have distinct roles during meiosis.