Progressive Differentiation and Instructive Capacities of Amniotic Fluid and Cerebrospinal Fluid Proteomes following Neural Tube Closure.
Progressive Differentiation and Instructive Capacities of Amniotic Fluid and Cerebrospinal Fluid Proteomes following Neural Tube Closure.
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DOI：
10.1016/j.devcel.2015.11.015
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发表时间：
2015-12-21
影响因子：
11.8
通讯作者：
Lehtinen MK
中科院分区：
文献类型：
--
作者：
Chau KF;Springel MW;Broadbelt KG;Park HY;Topal S;Lun MP;Mullan H;Maynard T;Steen H;LaMantia AS;Lehtinen MK
神经管闭合后，捕获在神经管内的羊水（AF）形成新生脑脊液（CSF）。神经上皮干细胞接触充满CSF的脑室，增殖和分化，形成哺乳动物的大脑，而神经原基板，产生颅感觉神经元，保持与AF接触。使用体内超声成像，我们量化了胚胎脑室CSF空间从一开始的扩张。我们开发了工具，以获得纯AF和新生CSF，神经管关闭之前和之后，并定义如何AF和CSF蛋白质组在小鼠发育过程中的分歧。使用胚胎神经外植体，我们表明，年龄匹配的液体促进Sox2阳性神经源性的身份在发展中的前脑和嗅上皮。新生CSF还刺激前脑祖细胞的Sox2阳性自我更新，其中一些可归因于LIFR信号传导。我们的资源应该有助于在这个非常脆弱的早期大脑发育阶段的液体组织相互作用的调查。
After neural tube closure, amniotic fluid (AF) captured inside the neural tube forms the nascent cerebrospinal fluid (CSF). Neuroepithelial stem cells contact CSF-filled ventricles, proliferate and differentiate to form the mammalian brain, while neurogenic placodes, which generate cranial sensory neurons, remain in contact with the AF. Using in vivo ultrasound imaging, we quantified the expansion of the embryonic ventricular-CSF space from its inception. We developed tools to obtain pure AF and nascent CSF, before and after neural tube closure, and define how the AF and CSF proteomes diverge during mouse development. Using embryonic neural explants, we demonstrate that age-matched fluids promote Sox2-positive neurogenic identity in developing forebrain and olfactory epithelia. Nascent CSF also stimulates Sox2-positive self-renewal of forebrain progenitor cells, some of which is attributable to LIFR signaling. Our resource should facilitate the investigation of fluid-tissue interactions during this highly vulnerable stage of early brain development.