Direct Electrochemical Measurements of Reactive Oxygen and Nitrogen Species in Nontransformed and Metastatic Human Breast Cells
Direct Electrochemical Measurements of Reactive Oxygen and Nitrogen Species in Nontransformed and Metastatic Human Breast Cells
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DOI：
10.1021/jacs.7b06476
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发表时间：
2017-09-20
影响因子：
15
通讯作者：
Mirkin, Michael V.
中科院分区：
文献类型：
--
作者：
Li, Yun;Hu, Keke;Mirkin, Michael V.
人体细胞中活性氧和氮（ROS 和 RNS）的产生与多种疾病有关，包括癌症。涂有铂黑的微米级电极和镀铂铂纳米电极此前已用于检测生物系统中的初级ROS和RNS。在本文中，我们报告了具有良好表征几何形状的铂碳纳米电极的开发，并将其用作扫描电化学显微镜 (SECM) 尖端来测量非癌性和转移性人类乳腺细胞内的 ROS 和 RNS。通过在不同电位下进行时间依赖性定量电流测量，确定了细胞质中四种关键 ROS/RNS 的相对浓度及其动态，并用于阐明非转化和转移性人乳腺细胞中 ROS/RNS 的化学起源和生产率。
The production of reactive oxygen and nitrogen species (ROS and RNS) in human cells is implicated in various diseases, including cancer. Micrometer-sized electrodes coated with Pt black and platinized Pt nanoelectrodes have previously been used for the detection of primary ROS and RNS in biological systems. In this Article, we report the development of platinized carbon nanoelectrodes with well-characterized geometry and use them as scanning electrochemical microscopy (SECM) tips to measure ROS and RNS inside noncancerous and Metastatic human breast cells. By performing time-dependent quantitative amperometric measurements at different potentials, the relative concentrations of four key ROS/RNS in the cell cytoplasm and their dynamics were determined and used to elucidate the chemical origins and production rates of ROS/RNS in nontransformed and metastatic human breast cells.