Acceleration of wound healing by combined gene transfer of hepatocyte growth factor and prostacyclin synthase with Shima Jet
Acceleration of wound healing by combined gene transfer of hepatocyte growth factor and prostacyclin synthase with Shima Jet
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DOI：
10.1038/sj.gt.3302767
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发表时间：
2006-08-01
期刊：
影响因子：
5.1
通讯作者：
Morishita, R.
中科院分区：
文献类型：
--
作者：
Kunugiza, Y.;Tomita, N.;Morishita, R.
皮肤病是基因治疗的靶病之一，但目前尚无实用的基因转移方法。首先，我们检测了弹簧动力喷射器Shima jet的基因转移效率，该喷射器最初是作为胰岛素的无针喷射器开发的。荧光素酶质粒用岛岛喷气机转移时，局部基因表达量比用针转移高100倍左右。β -半乳糖苷酶基因转移显示基因在表皮表达。基于这些结果，我们随后研究了使用Shima Jet进行伤口愈合的基因治疗的潜力。肝细胞生长因子（HGF）基因转染后，肉芽组织表皮细胞增殖增加，微血管数量增加。前列腺环素合成酶（PGIS）基因转移后，创面周围血流量增加。HGF基因转移组促进创面愈合，联合基因转移组进一步促进创面愈合。这些结果表明，通过Shima Jet将HGF和PGIS基因共同转移可能是伤口愈合的有效策略。
Although skin diseases are one of the target diseases for gene therapy, there has been no practical gene transfer method. First, we examined gene transfer efficiency of the spring-powered jet injector, Shima Jet, which was originally developed as a non-needle jet injector of insulin. Local gene expression was about 100 times higher when the luciferase plasmid was transferred by the Shima Jet than by a needle. Gene transfer of beta-galactosidase revealed gene expression in the epidermis. Based on these results, we then examined the potential of gene therapy using the Shima Jet for wound healing. An increase of cellular proliferation of the epidermis and the number of microvessels in the granulation tissue was observed after hepatocyte growth factor (HGF) gene transfer. An increase in blood flow around the wound was observed after prostacyclin synthase (PGIS) gene transfer. Moreover, promotion on wound healing was observed in HGF gene transferred group, and further promotion was observed in combined gene transferred group as assessed by measuring wound area. These results indicate that co-transfer of HGF and PGIS genes by the Shima Jet could be an effective strategy to wound healing.