A polygalacturonase of animal origin isolated from the root-knot nematode Meloidogyne incognita
A polygalacturonase of animal origin isolated from the root-knot nematode Meloidogyne incognita
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DOI：
10.1016/s0014-5793(02)02906-x
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发表时间：
2002-07-03
期刊：
影响因子：
3.5
通讯作者：
Rosso, MN
中科院分区：
文献类型：
--
作者：
Jaubert, S;Laffaire, JB;Rosso, MN
从植物寄生线虫南方根结线虫（Meloidogyne incognita）中克隆了第一个动物多聚半乳糖醛酸酶（PG，EC 2.1.15）的cDNA，命名为Mi-pg-1。在大肠杆菌中异源表达后，证实了MI-PG-1的酶活性。在MI-PG-1序列上存在预测的信号肽，以及MI-PG-1基因的转录本在感染性青少年的食道腺中的特异性定位，意味着MI-PG-1可以分泌到植物组织中。对MI-PG-1在寄生中的潜在作用进行了讨论。(C)2002年欧洲生物化学学会联合会。由Elsevier Science B. V.出版，版权所有。
The first animal polygalacturonase (PG, EC 2.1.15) encoding cDNA, Mi-pg-1, was cloned from the plant parasitic nematode Meloidogyne incognita. The enzymatic activity, of MI-PG-1 was confirmed after heterologous expression in Escherichia coli. The presence of a predicted signal peptide on the MI-PG-1 sequence together with the specific localization of the transcripts of the Mi-pg-1 gene in the oesophageal glands of infective juveniles imply that MI-PG-1 could be secreted into plant tissues. The potential role of MI-PG-1 in parasitism is discussed. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.