The role of histone acetylation in the allelic expression of the imprinted human insulin-like growth factor II gene
The role of histone acetylation in the allelic expression of the imprinted human insulin-like growth factor II gene
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DOI：
10.1006/bbrc.1998.9401
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发表时间：
1998-10-20
影响因子：
3.1
通讯作者：
Hoffman, AR
中科院分区：
文献类型：
--
作者：
Hu, JF;Oruganti, H;Hoffman, AR
可逆的组蛋白乙酰化在X染色体失活中起着重要作用。在动物基因组中，组蛋白在高度甲基化的失活X染色体上是低乙酰化的，而在未甲基化的“CpG岛”中是高乙酰化的。我们已经研究了组蛋白乙酰化是否参与了胰岛素样生长因子II（IGF2）的等位基因表达的调控，IGF2是一种母系印记基因。HSK 09是一种人成纤维细胞系，在培养物中保持IGF 2的正常单等位基因表达。当这些细胞用组蛋白去乙酰化酶抑制剂、丁酸钠或阿司他丁A处理时，从所有表达的启动子中观察到IGF2的双等位基因表达。这些结果表明，除了DNA甲基化，两个亲本等位基因的差异组蛋白乙酰化可能是另一个潜在的机制，IGF2的印记进行调节，可能通过改变染色体11p15上的印记位点的局部染色质结构。(C)北京：科学出版社.
Reversible histone acetylation plays a central role in X chromosome inactivation. Histones are hypoacetylated on the heavily methylated inactive X chromosome and are hyperacetylated in the unmethylated "CpG islands" in animal genomes. We have investigated whether histone acetylation is involved in the regulation of the allelic expression of insulin-like growth factor II (IGF2), a maternally imprinted gene. HSK09, a human fibroblast cell line, retained normal monoallelic expression of IGF2 in culture. When these cells were treated with histone deacetylase inhibitors, sodium butyrate or trichostatin A, biallelic expression of IGF2 was observed from all of the promoters that are expressed. These results suggest that, in addition to DNA methylation, differential histone acetylation of two parental alleles may be another potential mechanism by which the imprinting of IGF2 is regulated, probably through changes in the local chromatin structure of the imprinted locus on chromosome 11p15. (C) 1998 Academic Press.