METABOLISM AND TRANSLOCATION OF ALLANTOIN IN UREIDE-PRODUCING GRAIN LEGUMES
METABOLISM AND TRANSLOCATION OF ALLANTOIN IN UREIDE-PRODUCING GRAIN LEGUMES
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DOI：
10.1104/pp.70.2.476
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发表时间：
1982-01-01
期刊：
影响因子：
7.4
通讯作者：
PEOPLES, MB
中科院分区：
文献类型：
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作者：
ATKINS, CA;PATE, JS;PEOPLES, MB
以豇豆（Vigna unguiculata L.）离体嫩梢为材料，研究了尿囊素的N和C向叶片、茎和叶柄、茎尖、花梗、豆荚和种子中氨基酸和蛋白质的转移。哇CV. Caloona）植物[接种根瘤菌CB 756]在蒸腾流中供应[2- 14 C]、[1，3 - 15 N]尿囊素后表现出明显的生长抑制。在整个营养生长和生殖生长的所有植物器官表现出显着的尿素分解活性和容易代谢[2- 14 C]尿囊素14 CO2。酰脲氮的代谢途径是通过尿囊酸、尿素和氨来实现的。尿素分解活性水平的提取物从叶和根的pestulated豇豆始终保持在较高的水平比非pestulated，NO3-生长的植物。[14 C]脲提取物中回收的蚜虫（Aphis craccivora和Macrosiphum euphorbieae）饲养在豇豆植物上的不同位置，通过蒸腾流或单小叶的上表面供应[2- 14 C]尿囊素。酰脲能有效地从木质部或叶肉转移到韧皮部，然后在韧皮部中转移到果实、根尖和根中。
Transfer of the N and C of allantoin to amino acids and protein of leaflets, stems and petioles, apices, peduncles, pods, and seeds of detached shoots of nodulated cowpea (Vigna unguiculata L. Walp. cv. Caloona) plants [inoculated with Rhizobium CB756] was demonstrated following supply of [2-14C], [1,3-15N]allantoin in the transpiration stream. Throughout vegetative and reproductive growth all plant organs showed significant ureolytic activity and readily metabolized [2-14C]allantoin to 14CO2. A metabolic pathway for ureide N utilization via allantoic acid, urea and ammonia was indicated. Levels of ureolytic activity in extracts from leaves and roots of nodulated cowpea were consistently maintained at higher levels than in non-nodulated, NO3- grown plants. [14C]Ureides were recovered in extracts of aphids (Aphis craccivora and Macrosiphum euphorbieae) feeding at different sites on cowpea plants supplied with [2-14C]allantoin through the transpiration stream or to the upper surface of single leaflets. The ureides were effectively transferred from xylem or leaf mesophyll to phloem, and then translocated in phloem to fruits, apices and roots.