512-Channel and 13-Region Simultaneous Recordings Coupled with Optogenetic Manipulation in Freely Behaving Mice.
512-Channel and 13-Region Simultaneous Recordings Coupled with Optogenetic Manipulation in Freely Behaving Mice.
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DOI：
10.3389/fnsys.2016.00048
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发表时间：
2016
影响因子：
3
通讯作者：
Tsien JZ
中科院分区：
文献类型：
--
作者：
Xie K;Fox GE;Liu J;Tsien JZ
开发能够记录自由行为动物广泛脑回路的单单位活动和局部场电位（LFP）的技术是构建脑活动图的关键。虽然小鼠是最流行的哺乳动物遗传模型，但由于小鼠的体积小和头骨薄，体内神经记录传统上仅限于较小的通道数和较少的脑结构。在这里，我们描述了一个512通道四极系统，使我们能够同时记录十几个皮质和皮质下结构的行为小鼠。这项新技术提供了两个主要优势-即超低成本和自己动手的灵活性，可以针对许多大脑区域的任何组合。我们成功记录了小鼠大脑13个不同神经回路的单个单位和LFPs，包括前扣带皮层、压后皮层、体感皮层、次级听觉皮层、海马CA 1区、齿状回、下托、外侧内嗅皮层、嗅周皮层和前边缘皮层。这个512通道系统也可以与Cre-lox神经遗传学和光遗传学相结合，以进一步研究基因，细胞类型和电路动态之间的相互作用。最后，我们证明了复杂的刺激，如地震和恐惧引起的脚电击，触发了所有13个大脑区域的放电变化，支持神经代码高度分布的观点。此外，我们发现，在任何给定的大脑区域中进行局部光遗传学操纵都可能破坏网络振荡，并以全脑的方式引起单单位放电模式的变化，从而引起对光遗传学操纵行为的解释的警示。
The development of technologies capable of recording both single-unit activity and local field potentials (LFPs) over a wide range of brain circuits in freely behaving animals is the key to constructing brain activity maps. Although mice are the most popular mammalian genetic model, in vivo neural recording has been traditionally limited to smaller channel count and fewer brain structures because of the mouse’s small size and thin skull. Here, we describe a 512-channel tetrode system that allows us to record simultaneously over a dozen cortical and subcortical structures in behaving mice. This new technique offers two major advantages – namely, the ultra-low cost and the do-it-yourself flexibility for targeting any combination of many brain areas. We show the successful recordings of both single units and LFPs from 13 distinct neural circuits of the mouse brain, including subregions of the anterior cingulate cortices, retrosplenial cortices, somatosensory cortices, secondary auditory cortex, hippocampal CA1, dentate gyrus, subiculum, lateral entorhinal cortex, perirhinal cortex, and prelimbic cortex. This 512-channel system can also be combined with Cre-lox neurogenetics and optogenetics to further examine interactions between genes, cell types, and circuit dynamics across a wide range of brain structures. Finally, we demonstrate that complex stimuli – such as an earthquake and fear-inducing foot-shock – trigger firing changes in all of the 13 brain regions recorded, supporting the notion that neural code is highly distributed. In addition, we show that localized optogenetic manipulation in any given brain region could disrupt network oscillations and caused changes in single-unit firing patterns in a brain-wide manner, thereby raising the cautionary note of the interpretation of optogenetically manipulated behaviors.