Rapid loss of stress fibers in Chinese hamster ovary cells after hyperthermia.
Rapid loss of stress fibers in Chinese hamster ovary cells after hyperthermia.
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DOI：
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发表时间：
1985
期刊：
影响因子：
11.2
通讯作者：
J. R. Glass;R. DeWitt;A. Cress
中科院分区：
文献类型：
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作者：
J. R. Glass;R. DeWitt;A. Cress
本研究的目的是用罗丹明标记的鬼笔环肽（一种特异于F-肌动蛋白的探针）来表征高温（45度）对中国仓鼠卵巢细胞中肌动蛋白应力纤维分布的影响。荧光显微镜显示，在45度水浴中浸泡后，应力纤维迅速损失。在45度浸泡5分钟后，大约90%的细胞分析不包含可观察到的应力纤维。加热后在37度孵育细胞后可见应力纤维。恢复的应力纤维的外观发生蛋白质合成恢复，并在热处理后加入蛋白质合成抑制剂阻止了这些结构的再现。这些结果支持的假设，细胞骨架成分可能是一个目标的高温，解释了多变的生物效应的热量，特别是热辐射增敏。
This study was initiated to characterize the effect of hyperthermia (45 degrees) on the distribution of actin stress fibers in Chinese hamster ovary cells using rhodamine-conjugated phalloidin, a probe specific for F-actin. Fluorescent microscopy revealed a rapid loss of stress fibers after immersion in a 45 degrees water bath. After 5-min immersion at 45 degrees, approximately 90% of the cells analyzed did not contain observable stress fibers. Stress fibers were visible after incubation of cells at 37 degrees after heating. The recovery of the appearance of the stress fibers occurred as protein synthesis resumed, and addition of protein synthesis inhibitors following heat treatment blocked the reappearance of these structures. These results support the hypothesis that cytoskeletal components may be a target of hyperthermia, explaining the pleotropic biological effects of heat and, in particular, heat radiosensitization.