A simple statistical parameter for use in evaluation and validation of high throughput screening assays
A simple statistical parameter for use in evaluation and validation of high throughput screening assays
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DOI：
10.1177/108705719900400206
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发表时间：
1999-04-01
影响因子：
--
通讯作者：
Oldenburg, KR
中科院分区：
文献类型：
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作者：
Zhang, JH;Chung, TDY;Oldenburg, KR
准确和快速地从大型化学文库中鉴定活性化合物（“命中”）的能力一直是开发高通量筛选（HTS）测定的最终目标。从特定HTS测定中鉴定命中的能力在很大程度上取决于筛选中使用的测定的适用性或质量。用于评价HTS测定法用于命中鉴定的“适用性”的标准或参数尚未明确定义，因此仍然难以直接比较测定法的质量。在本报告中，定义了一个称为“Z因子”的筛选窗口系数。该系数反映了测定信号动态范围和与信号测量相关的数据变化，因此适用于测定质量评估。Z因子是每种HTS测定的无量纲、简单统计特征。Z因子为比较和评价测定质量提供了有用的工具，并可用于测定优化和验证。
The ability to identify active compounds ("hits") from large chemical libraries accurately and rapidly has been the ultimate goal in developing high-throughput screening (HTS) assays. The ability to identify hits from a particular HTS assay depends largely on the suitability or quality of the assay used in the screening. The criteria or parameters for evaluating the "suitability" of an HTS assay for hit identification are not well defined and hence it still remains difficult to compare the quality of assays; directly. In this report, a screening window coefficient, called "Z-factor," is defined. This coefficient is reflective of both the assay signal dynamic range and the data variation associated with the signal measurements, and therefore is suitable for assay quality assessment. The Z-factor is a dimensionless, simple statistical characteristic for each HTS assay. The Z-factor provides a useful tool for comparison and evaluation of the quality of assays, and can be utilized in assay optimization and validation.