Inhibitory activity of the human papillomavirus type 1 AU-rich element correlates inversely with the levels of the elav-like HuR protein in the cell cytoplasm
Inhibitory activity of the human papillomavirus type 1 AU-rich element correlates inversely with the levels of the elav-like HuR protein in the cell cytoplasm
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DOI：
10.1007/s007050050041
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发表时间：
2000-01-01
影响因子：
2.7
通讯作者：
Schwartz, S
中科院分区：
文献类型：
--
作者：
Carlsson, A;Schwartz, S
我们分析了一组粘膜和皮肤来源的上皮细胞中人乳头瘤病毒1型(HPV-1)mRNAs的晚期3‘非翻译区的负元件的活性。其抑制活性依赖于细胞系。对先前已被证明与HPV-1阴性RNA元件特异性相互作用的异质核糖核蛋白C1/C2和Hur水平的测定表明，细胞质部分中Hur蛋白的低水平与HPV-1元件的高抑制活性相关，而抑制活性低的细胞系在细胞质中含有高水平的Hur。这些结果表明，HUR蛋白对HPV-1晚期基因的表达有正向影响。
We have analysed the activity of the negative element in the late 3' untranslated region of the cutaneous human papillomavirus type 1 (HPV-1) mRNAs in a panel of epithelial cells, of both mucosal and cutaneous origin. The inhibitory activity was cell line dependent. Determination of the levels of heterogeneous ribonucleoprotein C1/C2 and HuR that have been shown previously to interact specifically with the HPV-1 negative RNA element revealed that low levels of the HuR protein in the cytoplasmic fraction correlated with high inhibitory activity of the HPV-1 element, whereas cell lines displaying low inhibitory activity contained high levels of HuR in the cytoplasm. These results suggested that the HuR protein has a positive effect on HPV-1 late gene expression.