Wool and grain dusts stimulate TNF secretion by alveolar macrophages in vitro.
Wool and grain dusts stimulate TNF secretion by alveolar macrophages in vitro.
复制标题
羊毛和谷物粉尘在体外刺激肺泡巨噬细胞分泌 TNF。
DOI：
--
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发表时间：
1996
影响因子：
4.9
通讯作者：
K. Donaldson
中科院分区：
文献类型：
--
作者：
D. Brown;K. Donaldson
OBJECTIVE: The aim of the study was to investigate the ability of two organic dusts, wool and grain, and their soluble leachates to stimulate secretion of tumour necrosis factor (TNF) by rat alveolar macrophages with special reference to the role of lipopolysaccharide (LPS). METHODS: Rat alveolar macrophages were isolated by bronchoalveolar lavage (BAL) and treated in vitro with whole dust, dust leachates, and a standard LPS preparation. TNF production was measured in supernatants with the L929 cell line bioassay. RESULTS: Both wool and grain dust samples were capable of stimulating TNF release from rat alveolar macrophages in a dose-dependent manner. The standard LPS preparation caused a dose-dependent secretion of TNF. Leachates prepared from the dusts contained LPS and also caused TNF release but leachable LPS could not account for the TNF release and it was clear that non-LPS leachable activity was present in the grain dust and that wool dust particles themselves were capable of causing release of TNF. The role of LPS in wool dust leachates was further investigated by treating peritoneal macrophages from two strains of mice, LPS responders (C3H) and LPS non-responders (C3H/HEJ), with LPS. The non-responder mouse macrophages produced very low concentrations of TNF in response to the wool dust leachates compared with the responders. CONCLUSIONS: LPS and other unidentified leachable substances present on the surface of grain dust, and to a lesser extent on wool dust, are a trigger for TNF release by lung macrophages. Wool dust particles themselves stimulate TNF. TNF release from macrophages could contribute to enhancement of inflammatory responses and symptoms of bronchitis and breathlessness in workers exposed to organic dusts such as wool and grain.
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影响因子：
4.4
作者：
F. Luscinskas;M. Cybulsky;J. Kiely;Christopher S. Peckins;V. Davis;M. Gimbrone
通讯作者：
F. Luscinskas;M. Cybulsky;J. Kiely;Christopher S. Peckins;V. Davis;M. Gimbrone
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DOI：
10.1172/jci115755
发表时间：
1992
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Adi,S;Pollock,AS;Shigenaga,JK;Moser,AH;Feingold,KR;Grunfeld,C
通讯作者：
Grunfeld,C
影响因子：
4.4
作者：
J. Pober;M. Gimbrone;L. Lapierre;D. Mendrick;W. Fiers;R. Rothlein;T. Springer
通讯作者：
J. Pober;M. Gimbrone;L. Lapierre;D. Mendrick;W. Fiers;R. Rothlein;T. Springer
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DOI：
--
发表时间：
1995
期刊：
The American journal of pathology.
影响因子：
--
作者：
Flory,CM;Jones,ML;Miller,BF;Warren,JS
通讯作者：
Warren,JS