A COMPONENT OF CALCIUM-ACTIVATED POTASSIUM CHANNELS ENCODED BY THE DROSOPHILA-SLO LOCUS
A COMPONENT OF CALCIUM-ACTIVATED POTASSIUM CHANNELS ENCODED BY THE DROSOPHILA-SLO LOCUS
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DOI：
10.1126/science.1857984
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发表时间：
1991-08-02
期刊：
影响因子：
56.9
通讯作者：
GANETZKY, B
中科院分区：
文献类型：
--
作者：
ATKINSON, NS;ROBERTSON, GA;GANETZKY, B
钙激活钾通道在电兴奋细胞中介导许多重要的生物学功能。尽管电压激活的 K+ 通道的分子分析最近取得了进展，但 Ca2+ 激活的 K+ 通道尚未得到类似的表征。果蝇 Slowpoke (slo) 基因座的突变专门消除了肌肉和神经元中 Ca2+ 激活的 K+ 电流，为这些通道的分子表征提供了机会。分离并测序来自 slo 基因座的基因组和互补 DNA 克隆。 slo 预测的多肽与已知对功能至关重要的离散结构域中的电压激活的 K+ 通道多肽相似。因此，这些结果表明 slo 编码 Ca2+ 激活的 K+ 通道的结构成分。
Calcium-activated potassium channels mediate many biologically important functions in electrically excitable cells. Despite recent progress in the molecular analysis of voltage-activated K+ channels, Ca2+-activated K+ channels have not been similarly characterized. The Drosophila slowpoke (slo) locus, mutations of which specifically abolish a Ca2+-activated K+ current in muscles and neurons, provides an opportunity for molecular characterization of these channels. Genomic and complementary DNA clones from the slo locus were isolated and sequenced. The polypeptide predicted by slo is similar to voltage-activated K+ channel polypeptides in discrete domains known to be essential for function. Thus, these results indicate that slo encodes a structural component of Ca2+-activated K+ channels.