Isolated Rat Epididymal Basal Cells Share Common Properties with Adult Stem Cells
Isolated Rat Epididymal Basal Cells Share Common Properties with Adult Stem Cells
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DOI：
10.1095/biolreprod.115.133967
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发表时间：
2015-11-01
影响因子：
3.6
通讯作者：
Cyr, Daniel G.
中科院分区：
文献类型：
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作者：
Mandon, Marion;Hermo, Louis;Cyr, Daniel G.
关于附睾基底细胞功能的信息很少。这些细胞分泌洋地黄素，可以代谢自由基氧，并具有作为血液-附睾屏障组成部分的顶端突起。本研究的目的是开发一种可重复的协议，以分离大鼠附睾基底细胞，并通过基因表达谱来表征其功能。整合素-α 6用于分离高度纯化的基底细胞群。微阵列分析表明，552个基因的表达水平富集在基底细胞相对于其他细胞类型。在这些基因中，有45个以5倍或更高的水平表达。这些高表达基因编码与细胞粘附、细胞骨架功能、离子转运、细胞信号传导和表皮功能有关的蛋白质，包括蛋白酶和抗蛋白酶、信号转导和转录因子。一些高表达的基因已被报道在成人干细胞，表明基底细胞可能代表附睾干细胞群。建立了基底细胞培养物，表明这些基底细胞可以在体外从角蛋白（KRT）5阳性细胞分化为表达KRT 8和连接蛋白26（柱状细胞的标志物）的细胞。这些数据提供了附睾基底细胞基因表达的新信息，并表明这些细胞可以作为成体干细胞。
There is little information on the function of epididymal basal cells. These cells secrete prostaglandins, can metabolize radical oxygen species, and have apical projections that are components of the blood-epididymis barrier. The objective of this study was to develop a reproducible protocol to isolate rat epididymal basal cells and to characterize their function by gene expression profiling. Integrin-alpha6 was used to isolate a highly purified population of basal cells. Microarray analysis indicated that expression levels of 552 genes were enriched in basal cells relative to other cell types. Among these genes, 45 were expressed at levels of 5-fold or greater. These highly expressed genes coded for proteins implicated in cell adhesion, cytoskeletal function, ion transport, cellular signaling, and epidermal function, and included proteases and antiproteases, signal transduction, and transcription factors. Several highly expressed genes have been reported in adult stem cells, suggesting that basal cells may represent an epididymal stem cell population. A basal cell culture was established that showed that these basal cells can differentiate in vitro from keratin (KRT) 5-positive cells to cells that express KRT8 and connexin 26, a marker of columnar cells. These data provide novel information on epididymal basal cell gene expression and suggest that these cells can act as adult stem cells.