Integrated analysis of phosphoproteome and ubiquitylome in epididymal sperm of buffalo (Bubalus bubalis).
Integrated analysis of phosphoproteome and ubiquitylome in epididymal sperm of buffalo (Bubalus bubalis).
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水牛（Bubalus bubalis）附睾精子磷酸化蛋白质组和泛素组的综合分析
DOI：
10.1002/mrd.23432
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发表时间：
2021-01
影响因子：
2.5
通讯作者：
Zhang M
中科院分区：
文献类型：
--
作者：
Zhang PF;Huang YL;Fu Q;He WT;Xiao K;Zhang M
In mammals, sperm need to mature in the epididymis to gain fertilization competency. However, the molecular mechanism underlying buffalo sperm maturation remains elusive. Exploring sperm physiology at the posttranslational modification (PTM) level could help to develop our understanding of these mechanisms. Protein phosphorylation and ubiquitination are major PTMs in the regulation of many biological processes. In the present study, to our knowledge, we report the first phosphoproteome and ubiquitylome of sperm collected from the caput, corpus, and cauda segments of the epididymis using liquid chromatography–mass spectrometry combined with affinity purification. In total, 647 phosphorylation sites in 294 proteins and 1063 ubiquitination sites in 446 proteins were characterized. Some of these proteins were associated with cellular developmental processes and energy metabolic pathways. Interestingly, 84 proteins were both phosphorylated and ubiquitinated, simultaneously. Some of these proteins were involved in, for example, spermatogenesis, reproduction, and spermatid development. Taken together, these data provide a theoretical basis for further functional analysis of phosphorylation and ubiquitination in epididymal sperm of buffalo and other mammals, and serve as an important resource for exploring the physiological mechanism underlying sperm maturation. Our study investigated the phosphoproteome and ubiquitylome of buffalo epididymal sperm for the first time. Our research develops our understanding of the molecular mechanisms underlying sperm maturation in buffalo.
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影响因子：
64.5
作者：
Huttlin EL;Jedrychowski MP;Elias JE;Goswami T;Rad R;Beausoleil SA;Villén J;Haas W;Sowa ME;Gygi SP
通讯作者：
Gygi SP
影响因子：
5.6
作者：
Baska, Kathleen M.;Manandhar, Gaurishankar;Sutovsky, Peter
通讯作者：
Sutovsky, Peter
DOI：
10.1093/bioinformatics/bts251
发表时间：
2012-07-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Jiao X;Sherman BT;Huang da W;Stephens R;Baseler MW;Lane HC;Lempicki RA
通讯作者：
Lempicki RA
影响因子：
11.8
作者：
Kissel, H;Georgescu, MM;Steller, H
通讯作者：
Steller, H
影响因子：
3.6
作者：
Kong, Milene;Diaz, Emilce S.;Morales, Patricio
通讯作者：
Morales, Patricio