Selective isolation of components from natural volatile oil by countercurrent chromatography with cyclodextrins as selective reagent
Selective isolation of components from natural volatile oil by countercurrent chromatography with cyclodextrins as selective reagent
复制标题
以环糊精为选择性试剂逆流色谱法选择性分离天然挥发油中的成分
DOI：
10.1016/j.chroma.2016.03.071
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发表时间：
2016
影响因子：
4.1
通讯作者：
Ying Yongfei
中科院分区：
文献类型：
--
作者：
Tong Shengqiang;Lu Mengxia;Chu Chu;Yan Jizhong;Huang Juan;Ying Yongfei
Selective separation of chemical components from seven kinds of volatile oil by countercurrent chromatography with three types of cyclodextrins as selective reagent was investigated in this work. Preparative separation of chemical components from volatile oil is generally quite challenging due to their extremely complexity of the composition. A biphasic solvent systemn-hexane-0.10 mol L−1cyclodextrin (1:1, v/v) was selected for separation of components from volatile oil and three types of cyclodextrins were investigated, including β-cyclodexrin, methyl-β-cyclodexrin and hydroxypropyl-β-cyclodexrin. All kinds of volatile oils are from seven kinds of traditional Chinese herb. Results showed that some chemical components could be well separated with high purity from each kind of volatile oil using different type of cyclodextrin as selective reagent. For example, germacrone and curcumenone could be selectively separated from volatile oil ofCurcumae Rhizomawith methyl-β-cyclodexrin and hydroxypropyl-β-cyclodexrin as selector respectively, and other five components were selectively separated from volatile oil ofChuanxiong Rhizoma,Myristicae Semen,Aucklandiae RadixandAngelicae Sinensis Radixby countercurrent chromatography with different cyclodexrin as selective reagent. Separation mechanism for separation of components from volatile oil by countercurrent chromatography with cyclodextrin as selective reagent was proposed. Peak resolution of the present separation method could be greatly influenced by the chemical compositions of volatile oil.
