A role for pectin de-methylesterification in a developmentally regulated growth acceleration in dark-grown Arabidopsis hypocotyls
A role for pectin de-methylesterification in a developmentally regulated growth acceleration in dark-grown Arabidopsis hypocotyls
复制标题
DOI：
10.1111/j.1469-8137.2010.03409.x
复制
发表时间：
2010-01-01
期刊：
影响因子：
9.4
通讯作者：
Hoefte, Herman
中科院分区：
文献类型：
--
作者：
Pelletier, Sandra;Van Orden, Juergen;Hoefte, Herman
P>我们专注于在黑暗生长的拟南芥下胚轴中发育调节的生长加速，以研究细胞壁代谢的变化在控制细胞伸长中的作用。为此，对解剖的黑暗生长的下胚轴进行了精确的转录组分析，傅立叶变换红外（FT-IR）显微光谱和运动学分析。使用纤维素合成抑制剂，我们表明，生长加速标志着一个发育过渡，在此期间，生长与纤维素合成脱钩。接下来，我们研究了在这种转变过程中发生的细胞变化。FT-IR显微光谱显示细胞壁组成的显着变化过程中，但不是之后，生长加速。转录组分析表明细胞壁重塑，特别是果胶修饰，在这种生长加速的作用。这是证实了过度表达的果胶甲酯酶抑制剂，这引起了延迟的生长acceleration.This研究表明，细胞伸长的加速标志着在黑暗中生长的下胚轴细胞的发育过渡，并支持果胶脱甲酯化在这个事件的时间的作用。
P>We focused on a developmentally regulated growth acceleration in the dark-grown Arabidopsis hypocotyl to study the role of changes in cell wall metabolism in the control of cell elongation.To this end, precise transcriptome analysis on dissected dark-grown hypocotyls, Fourier transform infrared (FT-IR) microspectroscopy and kinematic analysis were used.Using a cellulose synthesis inhibitor, we showed that the growth acceleration marks a developmental transition during which growth becomes uncoupled from cellulose synthesis. We next investigated the cellular changes that take place during this transition. FT-IR microspectroscopy revealed significant changes in cell wall composition during, but not after, the growth acceleration. Transcriptome analysis suggested a role for cell wall remodeling, in particular pectin modification, in this growth acceleration. This was confirmed by the overexpression of a pectin methylesterase inhibitor, which caused a delay in the growth acceleration.This study shows that the acceleration of cell elongation marks a developmental transition in dark-grown hypocotyl cells and supports a role for pectin de-methylesterification in the timing of this event.