Evaluation of anti-nociceptive and anti-inflammatory activities of the methanol extract of Holigarna caustica (Dennst.) Oken leaves
Evaluation of anti-nociceptive and anti-inflammatory activities of the methanol extract of Holigarna caustica (Dennst.) Oken leaves
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DOI：
10.1016/j.jep.2019.01.025
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发表时间：
2019-05-23
影响因子：
5.4
通讯作者：
Uddin, Shaikh Bokhtear
中科院分区：
文献类型：
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作者：
Adnan, Md.;Chy, Md. Nazim Uddin;Uddin, Shaikh Bokhtear
民族药理学相关性：Holigarna caustica（Dennst.）在传统医学中常用于治疗各种疼痛状况，如眼睛刺激、炎症、关节炎、皮肤病、割伤和创伤。材料与方法：用新鲜的H。caustica），干燥，并用甲醇（MEHC）萃取。使用化学诱导（乙酸和福尔马林试验）和热诱导（热板和尾部浸泡试验）疼痛模型对MEHC进行活动试验。为了确定MEHC的抗伤害活性背后的可能机制，使用阿片受体拮抗剂纳洛酮来评估在福尔马林、热板和尾部浸泡试验中阿片受体的参与，而在乙酸诱导的扭体试验中分别使用亚甲蓝和格列本脲来评估cGMP和ATP敏感性K+通道通路的参与。同时，使用角叉菜胶诱导的爪水肿模型来确定提取物的抗炎潜力。通过旷场试验评价探索和运动行为。各种生物活性化合物可能负责的抗伤害性和抗炎活性，确定使用GC-MS analysis.Results：MEHC显示出强大的，显着的和剂量依赖性的抗伤害性活性在所有化学诱导的和热诱导的疼痛模型在所有实验剂量。阿片受体与所观察到的抗伤害作用的关联通过使用纳洛酮得到证实。cGMP和ATP敏感性K+通道通路也被证明参与MEHC的抗伤害活性。此外，MEHC对角叉菜胶诱导的炎性水肿表现出剂量依赖性抑制作用。MEHC与小鼠的自发活动或运动反应的变化无关。在GC-MS分析，40个化合物被确定，其中12个是记录的生物活性化合物具有强效的镇痛和抗炎properties.Conclusions：我们目前的研究表明，MEHC具有强大的中枢和外周抗伤害性以及抗炎活性。这也可以得出结论，阿片受体以及cGMP和ATP敏感的K+通道途径参与MEHC的抗伤害性机制。本研究合理化的民族医学使用的H。caustica叶在各种痛苦的条件。
Ethnopharmacological relevance: Holigarna caustica (Dennst.) is commonly used in traditional medicine to treat a variety of painful conditions such as eye irritation, inflammation, arthritis, skin diseases, cuts and wounds.Aim of the study: The present study was undertaken to investigate the anti-nociceptive and anti-inflammatory activities of the methanol extract of H. caustica leaves and to elucidate its possible mechanism(s) of action.Materials and methods: Fresh leaves of H. caustica were collected, dried, and extracted with methanol (MEHC). MEHC was subjected to activity testing, using chemical-induced (acetic acid and formalin test) and heat-induced (hot plate and tail immersion test) pain models. To determine the possible mechanism behind the anti-nociceptive activity of MEHC, the opioid antagonist naltrexone was used to evaluate the involvement of opioid receptors in the case of formalin, hot plate and tail immersion tests, while the involvement of the cGMP and ATP-sensitive K+ channel pathways were assessed using methylene blue and glibenclamide respectively, in the acetic acid-induced writhing test. In parallel, the carrageenan-induced paw oedema model was used to determine the anti-inflammatory potential of the extract. Exploratory and motor behaviours were evaluated by the open-field test. Various bioactive compounds potentially responsible for the anti-nociceptive and anti-inflammatory activities were ascertained using GC-MS analysis.Results: MEHC showed strong, significant and dose-dependent anti-nociceptive activity in all chemical-induced and heat-induced pain models at all experimental doses. The association of opioid receptors with the observed anti-nociceptive effects was confirmed by using naltrexone. The cGMP and ATP-sensitive K+ channel pathway was also shown to be involved in the anti-nociceptive activity of MEHC. In addition, MEHC exhibited a dose dependent inhibition of inflammatory oedema induced by carrageenan. MEHC was not connected with changes in either the locomotor activity or motor responses of mice. In a GC-MS analysis, 40 compounds were identified, among which twelve are documented bioactive compounds with potent analgesic and anti-inflammatory properties.Conclusions: Our current study revealed that MEHC possesses strong central and peripheral anti-nociceptive as well as anti-inflammatory activity. It may also be concluded that both opioid receptors as well as the cGMP and ATP-sensitive K+ channel pathway are involved in the anti-nociceptive mechanism of MEHC. This study rationalizes the ethnomedicinal use of H. caustica leaves in various painful conditions.