Hemopoietic Histocompatibility (Hh-1) Antigens: Polymorphism and Mapping
Hemopoietic Histocompatibility (Hh-1) Antigens: Polymorphism and Mapping
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造血组织相容性 (Hh-1) 抗原：多态性和作图
DOI：
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发表时间：
1987
期刊：
影响因子：
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通讯作者：
C. David
中科院分区：
文献类型：
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作者：
R. Rembecki;M. Bennett;Vinay Kumar;C. David
辐射H-2杂合子F1杂交小鼠对亲本骨髓细胞（BMC）移植物的“杂交抗性”表明Hh-1抗原是遗传性的。H-2纯合性是BMC移植物最佳免疫原性所必需的。H-2 B /H-2 d淋巴瘤细胞是Hh-1无效的，但不表达H-2 d的变体表达Hh-1 B。这表明在H-2D处或附近的反式作用基因下调Hh-1抗原的表达。NK细胞介导BMC移植物排斥，免疫应答样基因以Hh-1决定子特异性方式控制排斥BMC移植物的能力。我们使用了一组排斥某些品系BMC移植物的受体小鼠，以确定待测试的新品系小鼠的Hh-1表型。当非重组H-2类型的独立来源进行了研究，六种模式的表达四个不同的Hh-1决定簇被检测到。与H-2相比，非常有限的多态性程度是有趣的，因为NK细胞不重排免疫球蛋白或T细胞受体基因，并且预期具有缺乏的受体多样性。60多只H-2重组小鼠中绝大多数具有H-2D或L预测的Hh-1等位基因。然而，在众多的H-2S/D重组子中，有两个菌株表达Hh-1等位基因，这与L.独特的D d /L B重组体B10.RQDB表达Hh-1表型，这是d单倍型的特征，表明Hh-1定位于H-2L的左侧。由于L B是H-2 DL上唯一的B单倍型I类分子，因此Hh-1 B不可能是I类基因。在另一种菌株中，H-2S是Hh-1的预测因子。更令人感兴趣的是几种菌株，它们表达的Hh-1表型不同于产生重组体的两种供体菌株。我们认为这些菌株代表Hh-1内重组体;此外，Hh-1内交叉事件并不罕见。涉及H-2 f的突变体表明Hh-1 f的结构基因与H-2连锁，并且似乎定位在调节基因的左侧（可能是E β的着丝粒）。其他内部Hh-1重组体表明，大多数单倍型的结构基因映射在H-2的S/D间隔中调控基因的左侧。
“Hybrid resistance” to grafts of parental strain bone marrow cells (BMC) by irradiated H-2 heterozygous Fl hybrid mice suggested that Hh-1 antigens were inherited recessively. H-2 homozygosity is required for optimal immunogenicity of BMC grafts. H-2 b /H-2 d lymphoma cells are Hh-1 null but variants which do not express H-2D d do express Hh-1 b . This suggests that trans-acting genes at or near H-2D down-regulate the expression of Hh-1 antigens. NK cells mediate BMC graft rejection and immune response-like genes control the ability to reject BMC grafts in an Hh-1 determinant-specific manner. We have used a panel of recipient mice which reject certain strain BMC grafts in order to determine the Hh-1 phenotype of new strains of mice to be tested. When non-recombinant H-2 types of independent origin were studied, six patterns of expression of four different Hh-1 determinants were detected. The extremely limited degree of polymorphism compared to H-2 is interesting because NK cells do not rearrange immunoglobulin or T cell receptor genes and are expected to possess a paucity of receptor diversity. The great majority of more than 60 intra-H-2 recombinant strain mice had Hh-1 alleles predicted by H-2D or L. However, of the many H-2S/D recombinants, two strains expressed Hh-1 alleles which did not correlate with L. A unique D d /L b recombinant, B10.RQDB, expresses the Hh-1 phenotype which is characteristic of the d haplotype, indicating that Hh-1 maps to the left of H-2L. Since the Lb is the only b haplotype class I molecule at H-2DL, Hh-1 b cannot be a class I gene. In another strain, H-2S is predictive of Hh-1. Even more interesting are several strains which express Hh-1 phenotypes different from both donor strains which generated the recombinant. We believe that these strains represent intra-Hh-1 recombinants; furthermore, intra- Hh-1 crossover events are not rare. Recombinants involving H-2 f indicate that the structural genes for Hh-1 f are linked to H-2 and appear to map to the left of the regulatory genes (possibly centromeric of E β ). Other intra-Hh-1 recombinants indicate that the structural genes of most haplotypes map just to the left of the regulatory genes in the S/D interval of H-2.
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F1 杂交淋巴瘤细胞 H-2 丢失变体上造血组织相容性抗原的表达：与转基因调控一致的证据。
DOI：
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发表时间：
1987
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Rembecki,RM;Bennett,M;Kumar,V;Potter,TA
通讯作者：
Potter,TA
鼠自然杀伤细胞不表达 T 细胞受体的 α、β 或 γ 链基因的功能转录本。
DOI：
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发表时间：
1986
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Tutt,MM;Kuziel,WA;HackettJr,J;Bennett,M;Tucker,PW;Kumar,V
通讯作者：
Kumar,V
scid 小鼠的自然杀伤细胞中 T 细胞受体基因不会重排或表达功能转录本。
DOI：
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发表时间：
1987
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Tutt,MM;Schuler,W;Kuziel,WA;Tucker,PW;Bennett,M;Bosma,MJ;Kumar,V
通讯作者：
Kumar,V
干扰素-α/β刺激小鼠骨髓移植的遗传抗性。
DOI：
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发表时间：
1985
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Afifi,MS;Kumar,V;Bennett,M
通讯作者：
Bennett,M