SFRP2 methylation in fecal DNA -: a marker for colorectal polyps
SFRP2 methylation in fecal DNA -: a marker for colorectal polyps
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DOI：
10.1007/s00384-007-0355-2
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发表时间：
2008-01-01
影响因子：
2.8
通讯作者：
Mueller, Hannes M.
中科院分区：
文献类型：
--
作者：
Oberwalder, Michael;Zitt, Marion;Mueller, Hannes M.
分泌型卷曲相关蛋白（SFRP）的DNA甲基化可在结直肠癌（CRC）组织、腺瘤组织和异常隐窝病灶中检测到，而在正常结直肠粘膜组织中，SFRP基因未甲基化。最近，我们的研究小组能够证明SFRP 2甲基化是粪便中鉴定CRC的最敏感的单一DNA标记。本研究的目的是阐明在增生性和腺瘤性结直肠息肉患者的粪便中是否可以发现粪便DNA中的SFRP 2甲基化。本研究包括被诊断为结直肠息肉或结肠镜检查阴性的患者。从粪便样品中分离DNA。通过MethyLight评估SFRP 2甲基化。检查来自68个个体的粪便样本的DNA含量;来自健康对照的23%的样本（26个中的6个）、来自增生性息肉患者的46%的样本（13个中的6个）和来自腺瘤患者的45%的样本（29个中的13个）为人类DNA阳性。SFRP 2甲基化在健康对照组中没有发现，在33%（6例中的2例）的增生性息肉患者和46%（13例中的6例）的腺瘤患者中发现。统计分析显示，从健康对照组到增生性息肉患者和腺瘤患者，SFRP 2甲基化的频率显著增加（P=0.028）。在目前的研究中，我们首次报道了从健康对照组到增生性息肉患者和腺瘤患者，粪便DNA中SFRP 2甲基化显著增加。SFRP 2甲基化可作为粪便腺瘤和结直肠癌筛查的分子标志物。
DNA methylation of secreted frizzled-related proteins (SFRPs) can be detected in colorectal cancer (CRC) tissue, in tissue of adenomas, and in aberrant crypt foci, whereas in normal colorectal mucosa tissue, SFRP genes are unmethylated. Recently, our study group was able to demonstrate SFRP2 methylation as the most sensitive single DNA-based marker in stool for identification of CRC. The purpose of this study was to clarify whether SFRP2 methylation in fecal DNA can be found in stool of individuals with hyperplastic and adenomatous colorectal polyps.Patients who were diagnosed with colorectal polyps or showed negative colonoscopy were included in this study. DNA from stool samples was isolated. SFRP2 methylation was assessed by means of MethyLight.Stool samples from 68 individuals were checked for DNA content; 23% of the samples (6 of 26) from healthy controls, 46% of the samples (6 of 13) from patients with hyperplastic polyps, and 45% of the samples (13 of 29) from patients with adenomas were positive for human DNA. SFRP2 methylation in stool samples was found in none of the healthy controls, in 33% (2 of 6) patients with hyperplastic polyps, and in 46% (6 of 13) patients with adenomas. Statistical analysis revealed that the frequency of SFRP2 methylation increased significantly (P=0.028) from healthy controls to patients with hyperplastic polyps and to patients with adenomas.In the current study, we report for the first time that SFRP2 methylation in fecal DNA increases significantly from healthy controls to patients with hyperplastic polyps and to patients with adenomas. SFRP2 methylation may serve as a marker for molecular stool-based adenoma and CRC screening.