12-oxo-phytodienoic acid triggers expression of a distinct set of genes and plays a role in wound-induced gene expression in Arabidopsis
12-oxo-phytodienoic acid triggers expression of a distinct set of genes and plays a role in wound-induced gene expression in Arabidopsis
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DOI：
10.1104/pp.105.067058
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发表时间：
2005-11-01
期刊：
影响因子：
7.4
通讯作者：
Ohta, H
中科院分区：
文献类型：
--
作者：
Taki, N;Sasaki-Sekimoto, Y;Ohta, H
茉莉酸（Jasmonic acid， JA）和茉莉酸甲酯（methyl jasmonate, MeJA）统称为JAs，调节植物的多种生理过程，包括对伤害的反应。最近的报道表明，JA的环戊酮前体，12-氧-植物二烯酸（OPDA）也可以诱导基因表达。然而，对opda依赖性基因表达的生理意义知之甚少。我们使用微阵列分析了大约21,500个拟南芥（拟南芥）基因，比较了JA， MeJA和OPDA处理的反应。尽管许多基因对OPDA和JAs都有相同的反应，但我们发现了一组基因（OPDA特异性反应基因[ORGs]），它们对OPDA有特异性反应，而对JAs没有特异性反应。ORGs主要编码信号成分、转录因子和应激反应相关基因。一半的ORGs是由伤害引起的。对缺乏JAs生物合成的突变体的分析表明，OPDA在伤害反应中作为信号分子起作用。与通过JAs传递的信号不同，OPDA信号是不依赖于CORONATINE的不敏感信号。这些结果表明，OPDA信号通路独立于JA/MeJA信号通路发挥作用，并且是拟南芥损伤反应所必需的。
Jasmonic acid (JA) and methyl jasmonate (MeJA), collectively known as JAs, regulate diverse physiological processes in plants, including the response to wounding. Recent reports suggest that a cyclopentenone precursor of JA, 12-oxo-phytodienoic acid (OPDA), can also induce gene expression. However, little is known about the physiological significance of OPDA-dependent gene expression. We used microarray analysis of approximately 21,500 Arabidopsis (Arabidopsis thaliana) genes to compare responses to JA, MeJA, and OPDA treatment. Although many genes responded identically to both OPDA and JAs, we identified a set of genes (OPDA-specific response genes [ORGs]) that specifically responded to OPDA but not to JAs. ORGs primarily encoded signaling components, transcription factors, and stress response-related genes. One-half of the ORGs were induced by wounding. Analysis using mutants deficient in the biosynthesis of JAs revealed that OPDA functions as a signaling molecule in the wounding response. Unlike signaling via JAs, OPDA signaling was CORONATINE INSENSITIVE 1 independent. These results indicate that an OPDA signaling pathway functions independently of JA/MeJA signaling and is required for the wounding response in Arabidopsis.