Dichotomous Role of Jasmonic Acid in Modulating Sorghum Defense Against Aphids
Dichotomous Role of Jasmonic Acid in Modulating Sorghum Defense Against Aphids
复制标题
DOI：
10.1094/mpmi-01-22-0005-r
复制
发表时间：
2022-09-01
影响因子：
3.5
通讯作者：
Louis, Joe
中科院分区：
文献类型：
--
作者：
Grover, Sajjan;Puri, Heena;Louis, Joe
茉莉酸（JA）的前体及其衍生物有助于植物对昆虫攻击的保护性免疫。然而，JA在高粱防御甘蔗蚜虫（Melanaphis sacchari）中的作用仍不清楚，甘蔗蚜虫是高粱生产的主要威胁。高粱SC 265，以前被确定为SCA抗性基因型高粱巢式关联作图创始人线，短暂增加JAat蚜虫喂养的早期阶段，并阻止蚜虫定居。利用电图技术对蚜虫取食行为的监测表明，SC265植株限制了刺吸式昆虫对韧皮部汁液的SCA取食。然而，外源施用JA削弱了抗性表型，并促进了蚜虫在SC265植物上的摄食和定殖。这进一步证实了高粱JA缺乏的植物，其中JA缺乏促进蚜虫定居，然而，它也减少了蚜虫从韧皮部汁液中取食，并减少SCA人口。外源茉莉酸可促进缺茉莉酸植株上蚜虫的取食和增殖，表明茉莉酸可促进蚜虫的生长发育。与野生型植物相比，SCA饲喂JA缺陷植物改变了糖代谢，提高了果糖和海藻糖的水平。此外，含果糖和海藻糖的蚜虫人工饲料抑制了蚜虫的生长和繁殖。我们的研究结果强调了一个以前未知的二分作用的JA，这可能有相反的影响，阻止蚜虫定居在早期阶段，并提高蚜虫增殖能力在后期阶段的蚜虫定植高粱植物。
The precursors and derivatives of jasmonic acid (JA) contribute to plant protective immunity to insect attack. However, the role of JA in sorghum (Sorghum bicolor) defense against sugarcane aphid (SCA) (Melanaphis sacchari), which is consideredamajor threat to sorghumproduction, remains elusive. SorghumSC265, previously identified as a SCA-resistant genotype among the sorghum nested association mapping founder lines, transiently increased JAat early stages of aphid feeding and deterred aphid settling. Monitoring of aphid feeding behavior using electropenetrography, a technique to unveil feeding process of piercingsucking insects, revealed that SC265 plants restricted SCA feeding from the phloem sap. However, exogenous application of JA attenuated the resistant phenotype and promoted improved aphid feeding and colonization on SC265 plants. This was further confirmed with sorghum JA-deficient plants, in which JA deficiency promoted aphid settling, however, it also reduced aphid feeding from the phloem sap and curtailed SCA population. Exogenous application of JA caused enhanced feeding and aphid proliferation on JA-deficient plants, suggesting that JA promotes aphid growth and development. SCA feeding on JA-deficient plants altered the sugar metabolism and enhanced the levels of fructose and trehalose compared with wild-type plants. Furthermore, aphid artificial diet containing fructose and trehalose curtailed aphid growth and reproduction. Our findings underscore a previously unknown dichotomous role of JA, which may have opposing effects by deterring aphid settling during the early stage and enhancing aphid proliferative capacity during later stages of aphid colonization on sorghum plants.