Increased prevalence of antibiotic-resistant E. coli in gulls sampled in Southcentral Alaska is associated with urban environments.
Increased prevalence of antibiotic-resistant E. coli in gulls sampled in Southcentral Alaska is associated with urban environments.
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DOI：
10.3402/iee.v6.32334
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发表时间：
2016
影响因子：
--
通讯作者：
Bonnedahl J
中科院分区：
文献类型：
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作者：
Atterby C;Ramey AM;Hall GG;Järhult J;Börjesson S;Bonnedahl J
抗生素耐药细菌给全球医疗保健服务系统带来挑战；然而，关于此类细菌在环境中的流行和传播的信息有限。本研究的目的是比较阿拉斯加中南部城市和偏远地区大型海鸥（Larus spp.）中抗生素耐药性细菌的流行情况，以推断野生动物抗生素耐药性与人为影响的栖息地之间的关联。大肠杆菌是从海鸥 (n = 160) 的粪便样本中培养出来的 (n = 115 个分离株)，这些样本是从偏远地区米德尔顿岛和基奈半岛的城市环境中收集的。对米德尔顿岛白翅鸥 (Larus glucescens) 粪便样本中的大肠杆菌进行筛查后发现，8% 的分离株对一种或多种抗生素具有耐药性，2% 的分离株对三种或多种抗生素具有耐药性。相比之下，从基奈半岛大型海鸥（即灰鸥、鲱鱼 [Larus argentatus] 和潜在的杂交海鸥）粪便样本中提取的大肠杆菌分离株中，55% 对一种或多种抗生素具有耐药性，22% 对三种或多种抗生素具有耐药性。此外，来自基奈半岛各地的总共 16% 的海鸥样本含有超广谱头孢菌素耐药大肠杆菌分离株（超广谱 β-内酰胺酶 [ESBL] 和质粒编码的 AmpC [pAmpC]），而米德尔顿岛则没有检测到产生 ESBL 或 pAmpC 的分离株。我们的研究结果表明，抗生素耐药性患病率的增加与阿拉斯加中南部的城市环境有关，并且可能受到人为影响的影响。需要进一步调查来评估候鸟如何维持和传播与人类和动物健康相关的耐药细菌。
Antibiotic-resistant bacteria pose challenges to healthcare delivery systems globally; however, limited information is available regarding the prevalence and spread of such bacteria in the environment. The aim of this study was to compare the prevalence of antibiotic-resistant bacteria in large-bodied gulls (Larus spp.) at urban and remote locations in Southcentral Alaska to gain inference into the association between antibiotic resistance in wildlife and anthropogenically influenced habitats. Escherichia coli was cultured (n=115 isolates) from fecal samples of gulls (n=160) collected from a remote location, Middleton Island, and a more urban setting on the Kenai Peninsula. Screening of E. coli from fecal samples collected from glaucous-winged gulls (Larus glaucescens) at Middleton Island revealed 8% of isolates were resistant to one or more antibiotics and 2% of the isolates were resistant to three or more antibiotics. In contrast, 55% of E. coli isolates derived from fecal samples collected from large-bodied gulls (i.e. glaucous, herring [Larus argentatus], and potentially hybrid gulls) on the Kenai Peninsula were resistant to one or more antibiotics and 22% were resistant to three or more antibiotics. In addition, total of 16% of the gull samples from locations on the Kenai Peninsula harbored extended-spectrum cephalosporin-resistant E. coli isolates (extended-spectrum beta-lactamases [ESBL] and plasmid-encoded AmpC [pAmpC]), in contrast to Middleton Island where no ESBL- or pAmpC-producing isolates were detected. Our findings indicate that increased prevalence of antibiotic resistance is associated with urban environments in Southcentral Alaska and presumably influenced by anthropogenic impacts. Further investigation is warranted to assess how migratory birds may maintain and spread antimicrobial-resistant bacteria of relevance to human and animal health.