Stripping analysis of nucleic acids at a heated carbon paste electrode.
Stripping analysis of nucleic acids at a heated carbon paste electrode.
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DOI：
10.1021/ac000286q
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发表时间：
2000-07
影响因子：
7.4
通讯作者：
Joseph Wang;P. Gründler;G. Flechsig;M. Jasinski;G. Rivas;E. Sahlin;J. L. L. Paz-J.-L.-L.-Paz-47599710
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文献类型：
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作者：
Joseph Wang;P. Gründler;G. Flechsig;M. Jasinski;G. Rivas;E. Sahlin;J. L. L. Paz-J.-L.-L.-Paz-47599710
研制了一种新的电加热碳糊电极，用于痕量核酸的吸附溶出测定。电加热电极上的电化学与吸附恒流溶出计时电位法的这种耦合为核酸的痕量测量提供了独特的优势。在沉积步骤期间应用升高的温度导致剥离信号的显著（4-34倍，取决于所应用的温度）增强。这种改善归因于加热电极处的累积步骤。电极表面附近的强制热对流有利于使用静态溶液，因此可以使用超小体积。使用32 ℃的电极温度和1分钟累积期间的静止溶液，响应在1-8 mg/L的测试范围内呈线性，检测限为0.5 mg/L。这种电极加热技术为涉及核酸热操作的各种应用提供了很大的希望。
A new electrically heated carbon paste electrode has been developed for performing adsorptive stripping measurements of trace nucleic acids. Such coupling of electrochemistry at electrically heated electrodes with adsorptive constant-current stripping chronopotentiometry offers distinct advantages for trace measurements of nucleic acids. The application of increased temperatures during the deposition step results in dramatic (4-34-fold, depending on temperature applied) enhancement of the stripping signal. Such improvement is attributed to the accumulation step at the heated electrode. Forced thermal convection near the electrode surface facilitates the use of quiescent solutions and hence of ultrasmall volumes. Using an electrode temperature of 32 degrees C and a quiescent solution during the 1-min accumulation, the response is linear over the 1-8 mg/L range tested, with a detection limit of 0.5 mg/L. Such electrode heating technology offers great promise for various applications involving thermal manipulations of nucleic acids.