Comparison of visually evoked local field potentials in isolated turtle brain: patterned versus blank stimulation.
Comparison of visually evoked local field potentials in isolated turtle brain: patterned versus blank stimulation.
复制标题
离体龟脑视觉诱发局部场电位的比较：图案刺激与空白刺激。
DOI：
10.1016/j.jneumeth.2009.12.005
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发表时间：
2010
影响因子：
3
通讯作者：
Gao,Jia-Hong
中科院分区：
文献类型：
--
作者：
Luo,Qingfei;Lu,Huo;Lu,Hanbing;Yang,Yihong;Gao,Jia-Hong
最近，海龟离体脑/眼标本被用作无血动物模型，用于检测视觉诱发神经元电流产生的磁共振成像(MRI)信号变化。本工作旨在确定在龟脑/眼准备中，为了获得更强的神经元反应，应该使用棋盘图案还是全场闪光(空白)刺激。在这项研究中获得的知识对于使用海龟脑/眼准备进行功能神经成像研究中优化视觉刺激方法是必不可少的。本研究测量并比较了棋盘闪光和全场闪光三种不同刺激间隔(ISIS=5、10和16s)对甲鱼视皮层和视顶盖的视觉诱发局域场电位(LFP)的影响。结果发现，大脑皮质和顶盖LFP信号对棋盘刺激的神经元适应行为与闪光刺激相似。此外，两种刺激的LFP峰值幅度(ISI=16s)无显著差异。这些结果表明，神经元对棋盘刺激的反应强度与闪光刺激相当。这两种刺激方法在使用海龟脑/眼准备的功能神经成像研究中应该是等效的。
Isolated turtle brain/eye preparation has recently been used as a bloodless animal model for detecting the magnetic resonance imaging (MRI) signal changes produced by visually evoked neuronal currents. The present work aims to determine whether checkerboard-patterned or full field flash (blank) stimulation should be used in order to achieve stronger neuronal responses in turtle brain/eye preparation. The knowledge gained in this study is essential for optimizing the visual stimulation methods in functional neuroimaging studies using turtle brain/eye preparation. In this study, visually evoked local field potentials (LFPs) were measured and compared in turtle visual cortex and optic tectum elicited by checkerboard and full field flash stimuli with three different inter-stimulus intervals (ISIs=5, 10, and 16s). It was found that the behavior of neuronal adaptation in the cortical and tectal LFP signals for checkerboard stimulation was comparable to flash stimulation. In addition, there was no significant difference in the LFP peak amplitudes (ISI=16s) between these two stimuli. These results indicate that the intensity of neuronal responses to checkerboard is comparable to flash stimulation. These two stimulation methods should be equivalent in functional neuroimaging studies using turtle brain/eye preparation.