Association of the class V myosin Myo4p with a localised messenger RNA in budding yeast depends on She proteins.
Association of the class V myosin Myo4p with a localised messenger RNA in budding yeast depends on She proteins.
复制标题
V 类肌球蛋白 Myo4p 与芽殖酵母中局部信使 RNA 的关联取决于 She 蛋白。
作者：
Sonja Münchow;Claus Sauter;R. Jansen
信使RNA的不对称分布是将特定蛋白质的合成定位于细胞中不同位点的普遍机制。虽然尚未证实，但有相当多的证据表明，mRNA定位是一个积极的过程，取决于细胞骨架运动蛋白的活动。迄今为止，唯一的马达蛋白与mRNA定位的特定作用是芽殖酵母V型肌球蛋白Myo 4p。Myo 4p是定位ASH 1 mRNA所必需的，编码一种转录抑制因子，对芽殖酵母中HO基因的差异表达和交配型转换至关重要。Myo 4p、肌动蛋白细胞骨架蛋白以及其他四种特定基因SHE 2-SHE 5的突变会破坏ASH 1 mRNA的子体特异性定位。为了了解Myo 4p是否直接参与mRNA转运，我们使用Myo 4p和ASH 1 mRNA的原位共定位和共沉淀来测试它们的相互作用。我们的研究结果表明Myo 4p和ASH 1 mRNA的关联依赖于另外两个参与ASH 1 mRNA定位的基因SHE 2和SHE 3的活性。这强烈表明Myo 4p肌球蛋白作为局部mRNA的转运蛋白的直接作用，令人信服地支持基于运动蛋白的mRNA定位的概念。
Asymmetric distribution of messenger RNAs is a widespread mechanism to localize synthesis of specific protein to distinct sites in the cell. Although not proven yet there is considerable evidence that mRNA localisation is an active process that depends on the activity of cytoskeletal motor proteins. To date, the only motor protein with a specific role in mRNA localisation is the budding yeast type V myosin Myo4p. Myo4p is required for the localisation of ASH1 mRNA, encoding a transcriptional repressor that is essential for differential expression of the HO gene and mating type switching in budding yeast. Mutations in Myo4p, in proteins of the actin cytoskeleton, and in four other specific genes, SHE2-SHE5 disrupt the daughter-specific localisation of ASH1 mRNA. In order to understand if Myo4p is directly participating in mRNA transport, we used in situ colocalisation and coprecipitation of Myo4p and ASH1 mRNA to test for their interaction. Our results indicate an association of Myo4p and ASH1 mRNA that depends on the activity of two other genes involved in ASH1 mRNA localisation, SHE2 and SHE3. This strongly suggests a direct role of Myo4p myosin as a transporter of localised mRNAs, convincingly supporting the concept of motor-protein based mRNA localisation.
影响因子：
4
作者：
B K Haarer;A. Petzold;S. H. Lillie;S. Brown
通讯作者：
B K Haarer;A. Petzold;S. H. Lillie;S. Brown
影响因子：
16
作者：
Bertrand, E;Chartrand, P;Long, RM
通讯作者：
Long, RM
果蝇卵子发生过程中细胞质运输和细胞骨架组织的体内分析：多步前定位途径的表征。
DOI：
10.1242/dev.125.18.3655
发表时间：
1998
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Theurkauf,WE;Hazelrigg,TI
通讯作者：
Hazelrigg,TI