Identification of a Stable Quantitative Trait Locus for Percentage Grains with White Chalkiness in Rice (Oryza sativa)
Identification of a Stable Quantitative Trait Locus for Percentage Grains with White Chalkiness in Rice (Oryza sativa)
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DOI：
10.1111/j.1744-7909.2011.01041.x
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发表时间：
2011-08-01
影响因子：
11.4
通讯作者：
Wan, Jianmin
中科院分区：
文献类型：
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作者：
Guo, Tao;Liu, Xiaolu;Wan, Jianmin
垩白度高是世界上许多水稻产区的主要问题，特别是杂交水稻(Oryza sativa L.)在中国。我们以前使用染色体片段置换系(CSSL)在8号染色体的G1149-R727区间显示了一个控制白垩率的主数量性状基因座(QTLqPGWC-8)。在此，我们从Asominori(作为轮回亲本)与IR24(作为供体亲本)杂交产生的CSSL中选择了含有QTLqPGWC-8等位基因的品系-CSSL50，该品系具有较高的垩白率，与Asominori显著不同。Asominori和CSSL50在淀粉粒数、直链淀粉含量(AAC)和碾磨品质上也存在显著差异，但在籽粒大小和千粒重(TGW)上差异不显著。利用CSSL50与Asominori杂交的BC4F2和BC4F3群体对qPGWC-8进行了精细定位。我们将该QTL的定位范围缩小到Indel标记8G-7和8G-9之间的142kb区域。QTLqPGWC-8可解释亲本间PGWC差异的50.9%。这些与qPGWC-8紧密连锁的标记有助于该QTL基因的克隆，对水稻品质优良品种的分子标记辅助选择具有一定的参考价值。
High chalkiness is a major problem in many rice-producing areas of the world, especially in hybrid rice (Oryza sativa L.) in China. We previously showed a major quantitative trait locus for the percentage of grains with white chalkiness (QTLqPGWC-8) in the interval G1149-R727 on chromosome 8 using a chromosome segment substitution line (CSSL). Here, we selected the line-CSSL50 harboring the QTLqPGWC-8 allele from the CSSLs derived from a cross between Asominori (as a recurrent parent) and IR24 (as a donor parent), which had higher percentage chalkiness, markedly different from that of Asominori. There were also significant differences in starch granules, appearance of amylose content (AAC) and milling qualities between Asominori and CSSL50, but not in grain size or thousand grain weight (TGW). The BC4F2 and BC4F3 populations from a cross between CSSL50 and Asominori were used for fine mapping of qPGWC-8. We narrowed down the location of this QTL to a 142 kb region between Indel markers 8G-7 and 8G-9. QTLqPGWC-8 accounted for 50.9% of the difference in PGWC between the parents. The markers tightly linked to qPGWC-8 should facilitate cloning of the gene underlying this QTL and will be of value for marker-assisted selection in breeding rice varieties with better grain quality.