MONOAMINE OXIDASES OF THE BRAINS AND LIVERS OF MACAQUE AND CERCOPITHECUS MONKEYS
MONOAMINE OXIDASES OF THE BRAINS AND LIVERS OF MACAQUE AND CERCOPITHECUS MONKEYS
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DOI：
10.1016/0014-4886(92)90055-u
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发表时间：
1992-02-01
影响因子：
5.3
通讯作者：
HARIK, SI
中科院分区：
文献类型：
--
作者：
RIACHI, NJ;HARIK, SI
我们研究了非洲绿猴、恒河猴和食蟹猴这三种猴子大脑的不同区域以及脑微血管和大血管、脉络丛和肝脏中两种形式的单胺氧化酶(MAO-A和MAO-B)。MAO的测定是通过化学计量的不可逆的特定[~3H]肾上腺素结合和测量几种底物的氧化速率来确定的。脑微血管和大血管MAO含量较低。在三种猴子的大脑中，局部脑MAO的差异不超过1倍，并且基底节的MAO高于大脑皮层或小脑。脉络丛中的MAO活性较低，而肝脏的MAO活性高于任何大脑样本。在我们研究的所有组织中，绝大多数MAO是MAO-B型，特异性的[~3H]肾上腺素结合与1-甲基-4-苯基-1，2，3，6-四氢吡啶的氧化速率有很好的相关性。这些结果表明，猴和人在脑MAO分布上具有明显的相似性。在这三种猴子中，非洲绿猴的脑微血管中MAO活性最低，这构成了血脑屏障，尽管每组观察的数量很少，无法对差异进行统计分析。
We assessed the two forms of monoamine oxidase (MAO), MAO-A and MAO-B, in discrete regions of the brain and in cerebral micro- and macrovessels, choroid plexus, and liver of three species of monkeys: African Green, rhesus, and cynomolgus. MAO was determined by specific [3H]pargyline binding which is stoichiometric and irreversible and by measuring the rate of oxidation of several substrates. Cerebral micro- and macrovessels had low MAO content. Regional brain MAO did not vary by more than one-fold in the brains of each of the three species of monkeys and was higher in the basal ganglia than in the cerebral cortex or cerebellum. MAO in the choroid plexus was low, while the liver had higher MAO activity than any of the brain samples. The vast majority of MAO in all the tissues that we examined was of the MAO-B type, and specific [3H]pargyline binding correlated well with the oxidation rate of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine. These results show marked similarities in brain MAO distribution between monkey and man. Of the three monkey species, the African Green monkey had the lowest MAO activity in its cerebral microvessels, which constitute the blood-brain barrier, although the small number of observations in each group did not allow statistical analyses of the differences.