PROGRESSIVE CHANGES IN ADRENERGIC, SEROTONERGIC, AND PEPTIDERGIC NERVES IN PROXIMAL COLON OF STREPTOZOTOCIN-DIABETIC RATS
PROGRESSIVE CHANGES IN ADRENERGIC, SEROTONERGIC, AND PEPTIDERGIC NERVES IN PROXIMAL COLON OF STREPTOZOTOCIN-DIABETIC RATS
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DOI：
10.1016/0016-5085(88)90356-3
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发表时间：
1988-11-01
期刊：
影响因子：
29.4
通讯作者：
BURNSTOCK, G
中科院分区：
文献类型：
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作者：
BELAI, A;LINCOLN, J;BURNSTOCK, G
应用免疫组织化学、生化和免疫组织化学方法，观察了链脲佐菌素诱导大鼠糖尿病后8、16和25wk时，糖尿病进展对大鼠近端结肠肾上腺素能、5-羟色胺能和肽能神经支配的影响。从目前的研究中，出现了两种不同的糖尿病反应。第一个反应涉及去甲肾上腺素和血管活性肠肽，表现为变性的迹象，在诱导糖尿病后16和25周，组织水平和递质的免疫反应开始增加，随后组织水平和神经纤维密度下降。第二种反应涉及5-羟色胺、P物质和降钙素基因相关肽，其特征是组织水平和递质的免疫反应发生变化，没有退变的迹象。第三个特征是抵抗糖尿病引起的改变，这表现在神经肽Y神经，其中既没有神经肽Y组织水平的变化，也没有免疫反应性的变化。似乎所描述的总体变化将对链脲佐菌素-糖尿病大鼠模型的肠道功能产生深远的影响，该模型可能与糖尿病人类有一些相似之处。
The effect of progression of diabetes on adrenergic, serotonergic, and peptidergic innervation of the proximal colon of the rat at 8, 16, and 25 wk after induction of diabetes with streptoxotocin was investigated using immunohistochemical, biochemical, and immunochemical methods. Two different responses to diabetes emerged from the present study. The first response, which involves noradrenaline and vasoactive intestinal peptide, was characterized by a sign of degeneration, where there was an initial increase in tissue level and immunoreactivity of the transmitters followed by a decrease in tissue level and density of nerve fibers at 16 and 25 wk after induction of diabetes. The second response, which involves 5-hydroxytryptamine, substance P, and calcitonin gene-related peptide, was characterized by changes in tissue level and immunoreactivity of the transmitters with no evidence of degeneration. The third feature was one of resistance to change due to diabetes, which was demonstrated by neuropeptide Y-containing nerves, where there was neither a change in tissue level of neuropeptide Y nor a change in immunoreactivity. It seems likely that overall changes described will have profound implications in the function of the gut in the streptozotocin-diabetic rat model that may have some parallels in diabetic humans.